• Sensitive detection of β-galactosidase activity in situ
• Complete ready-to-use assay system containing all the necessary reagents
• Results in less than 24 hours post-transfection
• Easy visualization of positive cells
• Direct assay of transfections in cell culture dishes or in tissues in vivo

The X-Gal Staining Assay Kit is designed for sensitive and low-background in situ detection of cells transfected with vectors carrying the lacZ gene. This simple and rapid assay is suitable for expression studies in cultured cells and tissues transfected in vivo. The β-galactosidase enzyme catalyzes the hydrolysis of X-gal (5-bromo-3-indoyl-β-D-galactopyranoside), a β-galactoside. Following fixation and incubation with the X-gal substrate, cells transfected with a β-galactosidase-expressing plasmid will appear blue. These blue cells can be easily visualized by microscopy.

Contents
The X-gal Staining Assay Kit provides all the reagents necessary for performing 50 assays in 60 mm dishes.
- Fixing Buffer (1 vial x 125 ml)
- 10X PBS (1 vial x 75 ml)
- Staining Buffer (1 vial x 125 ml)
- 25X X-Gal stock  (4 vials x 1 ml)

Shipping/Storage
The X-gal Staining Assay Kit is shipped frozen.
The X-gal stock solution should be stored at -20ºC.
All other components should be stored at 4ºC.
Stable for 6 months when stored properly.

X-Gal Staining Kit - 50 assays/60 mm dishes • A10300K