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SoluLyse Reagent for Bacteria
  • Higher yields of soluble proteins
  • No interference with affinity purification resins
  • Retain more protein activity
  • Low viscosity without enzyme or sonication.
  • Simple one-step protocol.
  • Compatible with protease inhibitors, salts, chelating agents and reducing agents

The SoluLyse™ Protein Extraction Reagent is a proprietary formulation of nonionic detergents that is optimized for the most efficient extraction of soluble proteins from bacterial cells. It allows perforation of bacterial cell wall without denaturing proteins, and there is no need for secondary treatment such as sonication or freeze-thaw.

Side-by-side comparisons between SoluLyze and another leading commercial lysis reagents showed that SoluLyse yields about 10-fold more soluble proteins. In addition, SoluLyse reagent does not compromise protein activities in cell lysates.

The optimization of in vitro high-throughput chemical lysis of Escherichia coli. Application to ACP domain of the polyketide synthase ppsC from Mycobacterium tuberculosis

"In our comparison study, SoluLyse reagent has shown to be the method with the highest correlation to sonication. Interestingly, widely used lysozyme and Bugbuster® reagent failed in most our cases to completely release the soluble protein. When sonication is inconvenient, or difficult to apply to many samples, we find SoluLyse® reagent to be an acceptable alternative for the ACP fragment proteins as well as many other constructs we have examined to date.”
Link http://pubmedcentralcanada.ca/pmcc/articles/PMC2855807/pdf/10969_2009_Article_9077.pdf
Authors: Pawel Listwan, Jean-Denis Pédelacq, Meghan Lockard, Carolyn Bell,1 Thomas C. Terwilliger, and Geoffrey S. Waldo J Struct Funct Genomics 2010 March; 11(1): 41–49.

The SoluLyse reagent is easy to use. Simply add it to a bacterial pellet and gently mix for 10 minutes. The cells will be disrupted and soluble proteins released. Insoluble cell debris can then be removed by centrifugation. SoluLyse is also useful for the preparation of high-purity inclusion bodies when the protein of interest is insoluble.
The SoluLyse Reagent is tested to be compatible with all popular purification methods, such as Ni and glutathione resins for purifying poly-His and GST-tagged proteins. The unique, mild formulation of SoluLyse offers minimized interference with the binding and elution processes and ensures superior purification results. In addition, the reagent is compatible with a variety of additives such as protease inhibitors and reducing agents and may be readily dialyzed if needed.

Contents
- SoluLyse Protein Extraction Reagent is supplied in 1X solution in 50mM NaHPO4, pH 7.4.
- SoluLyse Reagent in Tris Buffer is supplied in 1X solution in 20 mM Tris HCl, pH 7.5.

Storage
Store at room temperature or +4ºC upon receipt. Stable for 2 years when stored properly.



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SoluLyse Reagent for Bacteria
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SoluLyse Reagent for Bacteria

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  • Higher yields of soluble proteins
  • No interference with affinity purification resins
  • Retain more protein activity
  • Low viscosity without enzyme or sonication.
  • Simple one-step protocol.
  • Compatible with protease inhibitors, salts, chelating agents and reducing agents

The SoluLyse™ Protein Extraction Reagent is a proprietary formulation of nonionic detergents that is optimized for the most efficient extraction of soluble proteins from bacterial cells. It allows perforation of bacterial cell wall without denaturing proteins, and there is no need for secondary treatment such as sonication or freeze-thaw.

Side-by-side comparisons between SoluLyze and another leading commercial lysis reagents showed that SoluLyse yields about 10-fold more soluble proteins. In addition, SoluLyse reagent does not compromise protein activities in cell lysates.

The optimization of in vitro high-throughput chemical lysis of Escherichia coli. Application to ACP domain of the polyketide synthase ppsC from Mycobacterium tuberculosis

"In our comparison study, SoluLyse reagent has shown to be the method with the highest correlation to sonication. Interestingly, widely used lysozyme and Bugbuster® reagent failed in most our cases to completely release the soluble protein. When sonication is inconvenient, or difficult to apply to many samples, we find SoluLyse® reagent to be an acceptable alternative for the ACP fragment proteins as well as many other constructs we have examined to date.”
Link http://pubmedcentralcanada.ca/pmcc/articles/PMC2855807/pdf/10969_2009_Article_9077.pdf
Authors: Pawel Listwan, Jean-Denis Pédelacq, Meghan Lockard, Carolyn Bell,1 Thomas C. Terwilliger, and Geoffrey S. Waldo J Struct Funct Genomics 2010 March; 11(1): 41–49.

The SoluLyse reagent is easy to use. Simply add it to a bacterial pellet and gently mix for 10 minutes. The cells will be disrupted and soluble proteins released. Insoluble cell debris can then be removed by centrifugation. SoluLyse is also useful for the preparation of high-purity inclusion bodies when the protein of interest is insoluble.
The SoluLyse Reagent is tested to be compatible with all popular purification methods, such as Ni and glutathione resins for purifying poly-His and GST-tagged proteins. The unique, mild formulation of SoluLyse offers minimized interference with the binding and elution processes and ensures superior purification results. In addition, the reagent is compatible with a variety of additives such as protease inhibitors and reducing agents and may be readily dialyzed if needed.

Contents
- SoluLyse Protein Extraction Reagent is supplied in 1X solution in 50mM NaHPO4, pH 7.4.
- SoluLyse Reagent in Tris Buffer is supplied in 1X solution in 20 mM Tris HCl, pH 7.5.

Storage
Store at room temperature or +4ºC upon receipt. Stable for 2 years when stored properly.


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