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Proteomics Research
SoluBL21™ Competent E. coli
  • Express toxic proteins
  • Optimized E. coli strain for expressing insoluble proteins in soluble form
  • Simple and straightforward protocol
  • Compatible with all T7 promoter-based expression vectors
  • 2 White Papers (See tab above)

Genlantis is pleased to offer SoluBL21™ Competent E. coli**, a significantly improved BL21 host strain for toxic and soluble protein expression. Click on the DATA tab. Using a directed evolution approach, Genlantis scientists have developed a mutant strain of BL21 DE3 E. coli that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product.


Contents

C700200 Quantity
SoluBL21 Chemically Competent E. coli 10 x 50 ul
SOC Medium 5.0 ml
pUC19 Positive Control Plasmid 20 ul (500 pg/ul)
C700210 Quantity
SoluBL21 Electrocompetent E. coli 10 x 20 ul
SOC Medium 5.0 ml
pUC19 Positive Control Plasmid 20 ul (10 pg/ul)

Shipping

The SoluBL21 Chemically Competent E. coli are shipped frozen.
For maximum stability and long-term use, store the cells and pUC19 control plasmid at -70ºC upon receipt.
All components are guaranteed stable for 6 months when stored properly

** Patent Pending


New Citations

The RNA Methyltransferase Complex of WTAP, METTL3, and METTL14 Regulates Mitotic Clonal Expansion in Adipogenesis
Kobayashi, M., et al (2018) Mol. Cell. Biol. 38 (16): e00116-18. Link

Identification of Iridoid Synthases from Nepeta species: Iridoid cyclization does not determine nepetalactone stereochemistry.
Sherden, N.H., et al (2017) bioRxiv 179572: 1-34 Link

An oomycete effector protein induces shade avoidance in Arabidopsis and attenuates salicylate signaling by binding to host proteins of the RADICAL-INDUCED CELL DEATH1 family.
Wirthmueller, L., et al (2017) bioRxiv 137844: 1-58 Link

FKBP8 recruits LC3A to mediate Parkin-independent mitophagy.
Bhujabal, Z., et al (2017) EMBO Rep. 18(6): 947-961.Link

Drosophila Dalmatian combines sororin and shugoshin roles in establishment and protection of cohesion.
Yamada, T., et al (2017) EMBO J. 36(11): 1513–1527. Link

Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase.
Takahata, T., et al (2017) J. Virol. 91(1): e02003-16. Link

Ehrlichia chaffeensis TRP32 Is a Nucleomodulin That Directly Regulates Expression of Host Genes Governing Differentiation and Proliferation.
Farris, T.R., et al (2016) Infect. Immun. 84(11): 3182-3194. Link

Sideromimic Modification of Lactivicin Dramatically Increases Potency against Extensively Drug-Resistant Stenotrophomonas maltophilia Clinical Isolates.
Calvopiña, K., et al (2016) Antimicrob. Agents Chemother. 60(7): 4170-4175. Link

Globin X is a six-coordinate globin that reduces nitrite to nitric oxide in fish red blood cells.
Corti, P., et al (2016) Proc. Natl. Acad. Sci. 113(30): 8538-8543. Link

Cross-class metallo-β-lactamase inhibition by bisthiazolidines reveals multiple binding modes.
Hinchliffe, P., et al (2016) Proc. Natl. Acad. Sci. 113(26): E3745-54. Link

Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.
Ciric, D., et al (2015) J. Virol. 89(12): 6287-6293. Link

Disruption of adaptor protein 2µ (AP-2µ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung, S-Y., et al (2015) EMBO J. 34(21):2686-2702. Link

How Vacuolar Sorting Receptor Proteins Interact with Their Cargo Proteins: Crystal Structures of Apo and Cargo-Bound Forms of the Protease-Associated Domain from an Arabidopsis Vacuolar Sorting Receptor.
Luo, F., et al (2014) Plant Cell 26(9): 3693–3708. Link


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SoluBL21™ Competent E. coli
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SoluBL21™ Competent E. coli

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Description
Data
Video
White Papers
  • Express toxic proteins
  • Optimized E. coli strain for expressing insoluble proteins in soluble form
  • Simple and straightforward protocol
  • Compatible with all T7 promoter-based expression vectors
  • 2 White Papers (See tab above)

Genlantis is pleased to offer SoluBL21™ Competent E. coli**, a significantly improved BL21 host strain for toxic and soluble protein expression. Click on the DATA tab. Using a directed evolution approach, Genlantis scientists have developed a mutant strain of BL21 DE3 E. coli that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product.


Contents

C700200 Quantity
SoluBL21 Chemically Competent E. coli 10 x 50 ul
SOC Medium 5.0 ml
pUC19 Positive Control Plasmid 20 ul (500 pg/ul)
C700210 Quantity
SoluBL21 Electrocompetent E. coli 10 x 20 ul
SOC Medium 5.0 ml
pUC19 Positive Control Plasmid 20 ul (10 pg/ul)

Shipping

The SoluBL21 Chemically Competent E. coli are shipped frozen.
For maximum stability and long-term use, store the cells and pUC19 control plasmid at -70ºC upon receipt.
All components are guaranteed stable for 6 months when stored properly

** Patent Pending


New Citations

The RNA Methyltransferase Complex of WTAP, METTL3, and METTL14 Regulates Mitotic Clonal Expansion in Adipogenesis
Kobayashi, M., et al (2018) Mol. Cell. Biol. 38 (16): e00116-18. Link

Identification of Iridoid Synthases from Nepeta species: Iridoid cyclization does not determine nepetalactone stereochemistry.
Sherden, N.H., et al (2017) bioRxiv 179572: 1-34 Link

An oomycete effector protein induces shade avoidance in Arabidopsis and attenuates salicylate signaling by binding to host proteins of the RADICAL-INDUCED CELL DEATH1 family.
Wirthmueller, L., et al (2017) bioRxiv 137844: 1-58 Link

FKBP8 recruits LC3A to mediate Parkin-independent mitophagy.
Bhujabal, Z., et al (2017) EMBO Rep. 18(6): 947-961.Link

Drosophila Dalmatian combines sororin and shugoshin roles in establishment and protection of cohesion.
Yamada, T., et al (2017) EMBO J. 36(11): 1513–1527. Link

Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase.
Takahata, T., et al (2017) J. Virol. 91(1): e02003-16. Link

Ehrlichia chaffeensis TRP32 Is a Nucleomodulin That Directly Regulates Expression of Host Genes Governing Differentiation and Proliferation.
Farris, T.R., et al (2016) Infect. Immun. 84(11): 3182-3194. Link

Sideromimic Modification of Lactivicin Dramatically Increases Potency against Extensively Drug-Resistant Stenotrophomonas maltophilia Clinical Isolates.
Calvopiña, K., et al (2016) Antimicrob. Agents Chemother. 60(7): 4170-4175. Link

Globin X is a six-coordinate globin that reduces nitrite to nitric oxide in fish red blood cells.
Corti, P., et al (2016) Proc. Natl. Acad. Sci. 113(30): 8538-8543. Link

Cross-class metallo-β-lactamase inhibition by bisthiazolidines reveals multiple binding modes.
Hinchliffe, P., et al (2016) Proc. Natl. Acad. Sci. 113(26): E3745-54. Link

Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.
Ciric, D., et al (2015) J. Virol. 89(12): 6287-6293. Link

Disruption of adaptor protein 2µ (AP-2µ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung, S-Y., et al (2015) EMBO J. 34(21):2686-2702. Link

How Vacuolar Sorting Receptor Proteins Interact with Their Cargo Proteins: Crystal Structures of Apo and Cargo-Bound Forms of the Protease-Associated Domain from an Arabidopsis Vacuolar Sorting Receptor.
Luo, F., et al (2014) Plant Cell 26(9): 3693–3708. Link

Soluble Protein Data

Figure 1: (click to enlarge) Expression of Insoluble Proteins in SoluBL21 vs. standard BL21(DE3) E. coli

Figure 1 legend: A panel of 22 clones that produced little or no soluble protein in standard BL21(DE3) cells were induced side-by-side in BL21(DE3) and SoluBL-21 E. coli. These clones encoded for a wide range of proteins including: cell cycle proteins, ATP-binding proteins, transferases, isomerases, kinases, and repair enzymes from human and other mammalian sources. With the SoluBL21 strain, ~70% of previously insoluble proteins were expressed in soluble form. Examples of SoluBL21-expressed proteins are shown above.


With Genlantis' SoluBL21 strain, a major obstacle to effective protein expression in E. coli has been overcome for many proteins. This significant improvement should enable scientists to make progress in a wide range of applications quicker and far less expensively than in the past.

Toxic Protein Data

We have evaluated the SoluBL21 host to determine whether clones toxic in BL21(DE3) and BL21(DE3)pLysS would exhibit similar toxicity in this slower growing variant. We demonstrate here that in addition to improving the solubility profile of many recombinant proteins, the SoluBL21 strain also permits the establishment of many clones that are toxic to, and cannot be established in, BL21(DE3) and BL21(DE3)pLysS. Furthermore, we show that these toxic clones produce substantial quantities of soluble protein when induced. White Paper Link

Transformation of Plasmid DNAs (Toxic Clones) into standard BL21(DE3), BL21(DE3)pLysS and SoluBL21 E. coli

 

Soluble Protein Fractions of "Toxic" Clones in SoluBL21 (DE3) E. coli

Additional information in SoluBL21 E. coli for Toxic Clones white paper

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