Home  .  Transfection  .  siRNA Transfection

siRNA Transfection

siRNA TransfectionRNA interference (RNAi) has become an important tool for studying gene functions because it allows sequence specific gene suppression in a variety of organisms and cultured cells. RNAi is characterized by targeted mRNA degradation after introduction of sequence-specific double stranded RNAs (dsRNAs) into cells. Several studies indicate that RNAi is an evolutionarily conserved defense mechanism directed against invading viral genomes or aberrant transcription products. In vitro studies using Drosophila lysates revealed that 21-25 nucleotide small interfering RNA duplexes (siRNAs) are the mediators of gene silencing. These siRNAs are derived from processing of the dsRNA by an RNase III-like enzyme. The mechanism involves the recruitment of siRNAs into a multi-protein complex known as RNA Induced Silencing Complex (RISC), which interacts with the target RNA to mediate cleavage in a catalytic fashion.

Complex pools of siRNAs can be produced by incubating in vitro transcribed long double stranded RNAs (~500bp) with recombinant RNase III or recombinant human Dicer enzyme. These pools compare favorably with chemically synthesized siRNAs in terms of their effectiveness and off-target effects and they have been successfully applied to both large scale screens and functional studies of individual proteins.

Genlantis offers a series of RNAi products for achieving targeted gene suppression. These include the highly optimized GeneSilencer® siRNA Transfection Reagent for efficient and functional siRNA delivery as well as the Dicer and Turbo Dicer siRNA Generation kits and components for enzyme-mediated in vitro production of heterogeneous siRNA cocktails.

GeneSilencer<sup>&#0174;</sup> Transfection Reagent
siGuard&#8482; RNase Inhibitor
Turbo Dicer&#8482; siRNA Generation Kit
Dicer siRNA Generation Kit
Efficient transfection of siRNA into mammalian cells
Prevent degradation of siRNA samples by RNase
More Robust Than Ever Rapid 2-hour digestion of dsRNA in vitro
Use recombinant dicer enzyme to easily and economically generate functional siRNAs
Stay Updated
Join our mailing list today to receive all the latest updates and information!
Corporate Headquarters
  • Genlantis, Inc.
  • 11011 Torreyana Rd
  • San Diego, CA 92121
  • 1-888-428-0558
Shop With Confidence
This shopping cart employs the highest industry strength security: 256 bit SSL E-commerce by Yahoo! We accept Visa, Master Card, American Express, Discover, PayPal
© 2013 Genlantis Inc. All rights reserved. Yahoo! Store by Solid Cactus