PrimaPure™ Rat Cells

• Each lot functionally characterized
• Complete kits or cells only
• Corresponding media available
• Each lot tested negative for mycoplasma, bacteria, yeast, and fungi
• Specified population doublings guaranteed for each cell type with Genlantis media and reagents

RAOEC

PrimaPure Rat Aortic Endothelial Cells (RAOEC) are isolated from aorta of healthy adult rats. They are cryopreserved at third passage and can be cultured and propagated to sixth passage and beyond without losing their morphologic and phenotypic characteristics. RAOEC is a relevant in vitro model for correlating with in vivo studies in rat as an animal model. RAOEC have been used as a model for the study of Atherosclerosis and products synthesized by the endothelium. The interaction of aortic endothelium and smooth muscle cells regulate the level and distribution of glycosaminoglycans in the arterial wall, especially in the intima. Co-culture of aortic endothelial cells and smooth muscle cells provide an ideal in vitro model for studying the interaction between these two cells types.

RAOSMC

PrimaPure Rat Aortic Smooth Muscle Cells (RAOSMC) are derived from the tunica intima and tunica media of healthy, fibrous plaque-free rat aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Increased arterial smooth muscle cell mass are found in the intimal lesion of atherosclerosis. Rat aortic smooth muscle cells respond to various factors by cell proliferation and hypertrophy, which are prominent indicators of atherosclerosis in vascular diseases. RAOSMC are well suited for the study of large vessel smooth muscle cell growth and differentiation and serve as in vitro model in correlation with live rat models

RCF

PrimaPure Rat Cardiac Fibroblasts (RCF) are derived from the heart tissue of normal Sprague Dawley rats. They are cryopreserved at second passage and can be cultured and propagated at least 8 population doublings. Rat Cardiac Fibroblasts are known to play key roles in the development of cardiac disease and hypertrophy. The regulation of fibroblast growth after acute myocardial infarction was determined in RCF. Cardiac fibroblasts were examined as a potential source of pro-inflammatory cytokine IL-6 synthesis which had been implicated in the pathophysiology of heart disease. RCF have also been used to study the autocrine/paracrine capability mediated by vasodilator such as adernomedullin. RCF are used to investigate their role in local hormone metabolism. Nitric oxide (NO) production in RCF decreases cAMP accumulation largely by the cGMP-mediated activation of PDE2.  IL-1 beta-induced inducible NO synthase expression can trigger NO-dependent apoptosis in adult RCF.

Cell Type	Decription	Quantity	Cell Passages	Recomended Media	Catalog #
RAOEC	Rat Aortic Endothelial Cells	>500,000 cells	2nd	Rat Endothelial Growth Medium	SR30405 (cells only) SR30405K (Complete Kit*)
RAOSMC	Rat Aortic Smooth Muscle Cells	>500,000 cells	2nd	Rat Smooth Muscle Growth Medium	SR35405 (cells only) SR35405K (Complete Kit**)
RCF	Rat Cardiac Fibroblasts	>500,000 cells	2nd	Rat Fibroblast Growth Medium	SR30605 (cells only) SR30605K (Complete Kit***)

*Each Complete System contains an ampoule of cryopreserved RAOEC  (PB30405), 500 ml of Rat Endothelial Cell Growth Medium (PMR211500), 100 ml of Attachment Factor Solution (PR123100) and a Subculture Reagent Kit (PR090100K).

**Each Complete System contains an ampoule of cryopreserved RAOSMC  (PB35405), 500 ml of Rat Smooth Muscle Cell Growth Medium (PMR311500), and a Subculture Reagent Kit (PR090100K).

***Each Complete System contains an ampoule of cryopreserved RCF  (SR30605A), 500 ml of Rat Fibroblast Growth Medium (PMR116500), and a Subculture Reagent Kit (PR090100K).

SR30405-SR30405K RAOEC Manual (pdf)
SR30605A RCF Manual (pdf)
SR35405 RAOSMC Manual (pdf)
PMR116GS Rat Cardiac Fibroblast Growth Supplements (pdf)