phCMV1 Restriction Site List #sites -- Bp position of recognition site -- AatII 4 121, 174, 257, 443 Acc65I 1 869 AccI 2 822, 863 AciI 61 (not expanded) AflII 1 1145 AflIII 1 4181 AluI 14 565, 841, 847, 1041 1208, 1465, 1760, 2048 2368, 2826, 3623, 3880 3926, 4016 Alw44I 1 3867 AlwI 14 642, 884, 885, 2123 2434, 2513, 2680, 3057 3072, 3431, 3444, 3529 3541, 3615 AlwNI 2 3359, 3767 ApaI 1 877 ApoI 4 853, 1077, 1181, 1192 AvaI 2 837, 880 AvaII 3 637, 2779, 3224 BamHI 1 884 BanI 6 465, 813, 869, 1416 2262, 2297 BanII 6 564, 840, 877, 1450 2624, 3334 BbeI 1 2262 BbsI 1 627 BbvI 13 1039, 1558, 2223, 2324 2366, 2407, 2500, 2887 3340, 3566, 3772, 3775 3840 BciVI 3 1681, 2471, 3972 BfaI 7 918, 1112, 1530, 2086 3101, 3435, 3688 BglI 5 84, 206, 277, 648 2032 BglII 1 833 BlnI 2 2085, 3100 BpmI 3 592, 3061, 3082 BsaAI 3 338, 1377, 2565 BsaBI 2 921, 2118 BsaHI 8 121, 174, 257, 443 597, 2262, 2964, 3043 BsaI 1 3258 BsaJI 19 360, 652, 796, 873 880, 1797, 1869, 1992 2027, 2036, 2085, 2426 2695, 3100, 3227, 3228 3330, 3331, 4021 BsaWI 4 2294, 3138, 3828, 3975 BseMII 6 1743, 2045, 2926, 3375 3498, 3907 BseRI 1 2066 BseSI 4 877, 2205, 2298, 3867 BsiEI 4 655, 907, 2169, 3844 BsiHKAI 5 564, 840, 2372, 2562 3867 BslI 20 43, 652, 791, 1269 1595, 1987, 2238, 2782 3063, 3095, 3096, 3227 3262, 3312, 3313, 3344 3697, 3976, 4142, 4160 BsmAI 7 429, 595, 778, 1693 2111, 3130, 3258 BsmBI 1 595 BsmFI 11 161, 312, 480, 636 1794, 1866, 1930, 2400 2932, 3041, 3225 BsmI 2 1008, 1107 Bsp1286I 11 564, 840, 877, 1450 2205, 2298, 2372, 2562 2624, 3334, 3867 BspHI 2 1689, 3461 BspMI 3 2159, 2522, 2972 BsrBI 4 1521, 1685, 2874, 2928 BsrDI 1 2489 BsrI 8 295, 1292, 1970, 2202 2403, 3649, 3766, 3779 BssKI 22 89, 282, 591, 634 658, 796, 880, 881 891, 1798, 1853, 1870 2266, 2426, 2649, 3039 3228, 3331, 3803, 4021 4034, 4155 BssSI 3 2855, 2981, 4008 Bst4CI 17 74, 196, 267, 541 579, 664, 785, 816 867, 1352, 2641, 2757 2865, 3188, 3679, 4149 4220 BstAPI 3 1824, 1896, 3358 BstBI 2 850, 2944 BstDSI 5 360, 652, 873, 1992 2695 BstF5I 6 602, 1950, 2117, 2574 2599, 3053 BstUI 18 59, 653, 678, 874 911, 1186, 1562, 1582 1606, 1637, 2328, 2629 3067, 3151, 3214, 3283 3556, 4137 Bsu36I 2 1742, 3374 Cac8I 29 84, 277, 810, 875 1480, 1523, 1537, 1810 1829, 1882, 1901, 2155 2341, 2560, 2626, 2632 2660, 2664, 2705, 2709 2763, 3046, 3233, 3280 3336, 3358, 3569, 4129 4166 Cfr10I 4 1480, 2582, 2763, 3046 ClaI 1 2102 Csp6I 10 218, 243, 298, 331 382, 539, 708, 870 895, 2568 CviRI 20 668, 750, 809, 859 1011, 1038, 1106, 1823 1832, 1895, 1904, 2019 2095, 2154, 2313, 2488 2502, 3572, 3868, 4235 CviTI 78 (not expanded) DdeI 6 1743, 2045, 2926, 3375 3498, 3907 DpnI 21 586, 642, 834, 885 921, 2101, 2105, 2124 2435, 2513, 2594, 2603 2681, 3057, 3073, 3432 3444, 3522, 3530, 3541 3616 DraI 3 963, 3405, 3424 DraIII 1 1374 DrdI 3 1328, 2284, 4073 EaeI 6 655, 907, 2169, 2343 2734, 2761 EarI 3 1735, 2612, 2822 EciI 9 506, 1747, 1944, 1965 1975, 1986, 2776, 3963 4109 Ecl136II 2 564, 840 Eco52I 3 655, 907, 2169 Eco57I 3 2388, 2820, 3654 EcoO109I 1 3360 EcoRI 1 853 EcoRII 13 89, 282, 591, 796 1798, 1853, 1870, 2649 3228, 3331, 4021, 4034 4155 EheI 1 2262 FauI 14 85, 111, 278, 875 1523, 1577, 1932, 1953 2393, 2930, 3068, 3149 3215, 3281 Fnu4HI 31 654, 906, 909, 1039 1558, 1580, 1594, 2032 2171, 2223, 2234, 2324 2329, 2366, 2407, 2494 2497, 2500, 2736, 2832 2873, 2887, 3001, 3340 3355, 3566, 3772, 3775 3840, 3983, 4138 FokI 6 602, 1950, 2117, 2574 2599, 3053 FspI 1 2363 HaeII 4 1526, 1534, 2262, 3937 HaeIII 22 83, 276, 656, 878 908, 1228, 1370, 2025 2031, 2040, 2083, 2170 2344, 2735, 2762, 3271 3328, 3361, 3706, 4140 4158, 4169 HgaI 7 517, 597, 1607, 2964 3043, 3502, 4080 HhaI 20 1527, 1535, 1561, 1583 1592, 1605, 1636, 2255 2263, 2327, 2364, 2630 2890, 3066, 3152, 3555 3664, 3838, 3938, 4005 HincII 3 822, 863, 1024 HindIII 1 846 HinfI 15 410, 682, 720, 765 914, 1306, 1328, 1738 2748, 2882, 2934, 2992 3029, 3811, 4207 HinP1I 20 1527, 1535, 1561, 1583 1592, 1605, 1636, 2255 2263, 2327, 2364, 2630 2890, 3066, 3152, 3555 3664, 3838, 3938, 4005 HpaI 1 1024 HphI 5 364, 1388, 2449, 3322 3438 KasI 1 2262 KpnI 1 869 MaeII 15 122, 134, 175, 258 339, 444, 702, 1323 1335, 1378, 1488, 2379 2566, 3347, 3480 MaeIII 13 62, 149, 498, 699 1052, 1553, 1565, 2384 2690, 3380, 3646, 3762 3825 MboII 10 627, 1507, 1735, 2612 2822, 2922, 3089, 3289 3441, 3532 MnlI 26 548, 619, 649, 949 972, 981, 1414, 1745 2023, 2029, 2036, 2042 2066, 2069, 2081, 2121 2185, 2321, 2678, 2854 3060, 3108, 3374, 3744 4011, 4068 MscI 1 2343 MslI 2 361, 2696 MspA1I 6 652, 873, 1759, 2367 3596, 3841 MspI 20 634, 658, 881, 891 1481, 2168, 2245, 2267 2295, 2426, 2516, 2583 2764, 3039, 3047, 3139 3613, 3803, 3829, 3976 MunI 1 1013 MvaI 13 89, 282, 591, 796 1798, 1853, 1870, 2649 3228, 3331, 4021, 4034 4155 MwoI 30 84, 206, 238, 277 370, 394, 600, 648 1484, 1514, 1541, 1583 1585, 1824, 1896, 1947 2026, 2032, 2248, 2332 2355, 2494, 2500, 2617 2700, 2967, 3154, 3358 3557, 4129 NaeI 3 1480, 2763, 3046 NarI 1 2262 NciI 9 634, 658, 880, 881 891, 2266, 2426, 3039 3803 NcoI 3 360, 1992, 2695 NdeI 1 233 NgoMIV 3 1480, 2763, 3046 NlaIII 16 301, 361, 752, 1690 1830, 1902, 1993, 2134 2479, 2665, 2696, 2722 3078, 3462, 4182, 4233 NlaIV 19 465, 636, 813, 869 877, 884, 1416, 1437 1449, 1640, 1801, 1873 2262, 2297, 3145, 3224 3269, 4112, 4151 NotI 1 906 NsiI 4 749, 1831, 1903, 4234 NspI 4 1829, 1901, 2664, 4181 PciI 1 4181 PleI 9 410, 720, 765, 914 1306, 1328, 1738, 2934 3811 Ppu10I 4 749, 1831, 1903, 4234 PsiI 2 1044, 1249 PspOMI 1 877 PstI 2 808, 858 PvuII 2 1759, 2367 RsaI 10 218, 243, 298, 331 382, 539, 708, 870 895, 2568 RsrII 1 2778 SacI 2 564, 840 SacII 2 652, 873 SalI 2 822, 863 SapI 2 2612, 2822 Sau3AI 21 586, 642, 834, 885 921, 2101, 2105, 2124 2435, 2513, 2594, 2603 2681, 3057, 3073, 3432 3444, 3522, 3530, 3541 3616 Sau96I 11 83, 276, 637, 877 878, 1370, 2779, 3224 3270, 3328, 3361 SchI 9 410, 720, 765, 914 1306, 1328, 1738, 2934 3811 ScrFI 22 89, 282, 591, 634 658, 796, 880, 881 891, 1798, 1853, 1870 2266, 2426, 2649, 3039 3228, 3331, 3803, 4021 4034, 4155 SexAI 1 1852 SfaNI 11 367, 1070, 1833, 1905 2231, 2486, 2553, 2617 2702, 2892, 4094 SfcI 8 715, 724, 771, 808 858, 1598, 3725, 3916 SfiI 1 2031 SmaI 1 880 SmlI 7 837, 843, 1145, 2654 3536, 3813, 4075 SnaBI 1 338 SphI 3 1829, 1901, 2664 SspI 2 1169, 1722 StuI 1 2082 StyI 5 360, 1992, 2085, 2695 3100 TaiI 15 122, 134, 175, 258 339, 444, 702, 1323 1335, 1378, 1488, 2379 2566, 3347, 3480 TaqI 15 823, 838, 851, 864 1412, 2103, 2376, 2532 2556, 2592, 2754, 2945 2990, 3245, 4083 TatI 4 217, 297, 330, 381 TfiI 6 682, 2748, 2882, 2992 3029, 4207 Tru9I 17 7, 758, 828, 964 1025, 1146, 1167, 1178 1190, 1201, 1218, 1316 1587, 3406, 3420, 3425 3477 TseI 13 1039, 1558, 2223, 2324 2366, 2407, 2500, 2887 3340, 3566, 3772, 3775 3840 Tsp45I 4 699, 1553, 2384, 2690 Tsp509I 12 19, 756, 854, 1014 1078, 1156, 1182, 1193 1840, 1912, 2004, 3422 TspRI 5 1103, 2386, 3496, 3767 3780 Tth111I 1 2378 VspI 2 6, 757 XbaI 1 917 XhoI 1 837 XhoII 9 833, 884, 2434, 2680 3072, 3431, 3443, 3529 3540 XmaI 1 880 phCMV2 – Restriction Sites #sites -- Bp position of recognition site -- AatII 4 121, 174, 257, 443 Acc65I 1 891 AccI 2 805, 885 AccIII 1 834 AciI 61 (not expanded) AflII 1 1167 AflIII 1 4203 AluI 14 565, 863, 869, 1063 1230, 1487, 1782, 2070 2390, 2848, 3645, 3902 3948, 4038 Alw44I 1 3889 AlwI 14 642, 906, 907, 2145 2456, 2535, 2702, 3079 3094, 3453, 3466, 3551 3563, 3637 AlwNI 2 3381, 3789 ApaI 1 899 ApoI 4 875, 1099, 1203, 1214 AvaI 2 859, 902 AvaII 3 637, 2801, 3246 BamHI 1 906 BanI 6 465, 799, 891, 1438 2284, 2319 BanII 6 564, 862, 899, 1472 2646, 3356 BbeI 1 2284 BbsI 1 627 BbvI 13 1061, 1580, 2245, 2346 2388, 2429, 2522, 2909 3362, 3588, 3794, 3797 3862 BciVI 3 1703, 2493, 3994 BfaI 8 845, 940, 1134, 1552 2108, 3123, 3457, 3710 BglI 5 84, 206, 277, 648 2054 BglII 1 855 BlnI 2 2107, 3122 BpmI 3 592, 3083, 3104 BsaAI 3 338, 1399, 2587 BsaBI 2 943, 2140 BsaHI 8 121, 174, 257, 443 597, 2284, 2986, 3065 BsaI 1 3280 BsaJI 19 360, 652, 782, 895 902, 1819, 1891, 2014 2049, 2058, 2107, 2448 2717, 3122, 3249, 3250 3352, 3353, 4043 BsaWI 5 834, 2316, 3160, 3850 3997 BseMII 6 1765, 2067, 2948, 3397 3520, 3929 BseRI 1 2088 BseSI 4 899, 2227, 2320, 3889 BsiEI 4 655, 929, 2191, 3866 BsiHKAI 5 564, 862, 2394, 2584 3889 BslI 20 43, 652, 777, 1291 1617, 2009, 2260, 2804 3085, 3117, 3118, 3249 3284, 3334, 3335, 3366 3719, 3998, 4164, 4182 BsmAI 6 429, 595, 1715, 2133 3152, 3280 BsmBI 1 595 BsmFI 11 161, 312, 480, 636 1816, 1888, 1952, 2422 2954, 3063, 3247 BsmI 2 1030, 1129 Bsp1286I 11 564, 862, 899, 1472 2227, 2320, 2394, 2584 2646, 3356, 3889 BspHI 2 1711, 3483 BspMI 3 2181, 2544, 2994 BsrBI 4 1543, 1707, 2896, 2950 BsrDI 1 2511 BsrI 8 295, 1314, 1992, 2224 2425, 3671, 3788, 3801 BssKI 22 89, 282, 591, 634 658, 782, 902, 903 913, 1820, 1875, 1892 2288, 2448, 2671, 3061 3250, 3353, 3825, 4043 4056, 4177 BssSI 3 2877, 3003, 4030 Bst4CI 17 74, 196, 267, 541 579, 664, 771, 802 889, 1374, 2663, 2779 2887, 3210, 3701, 4171 4242 BstAPI 3 1846, 1918, 3380 BstBI 2 872, 2966 BstDSI 5 360, 652, 895, 2014 2717 BstF5I 6 602, 1972, 2139, 2596 2621, 3075 BstUI 18 59, 653, 678, 896 933, 1208, 1584, 1604 1628, 1659, 2350, 2651 3089, 3173, 3236, 3305 3578, 4159 Bsu36I 2 1764, 3396 Cac8I 31 84, 277, 751, 796 844, 897, 1502, 1545 1559, 1832, 1851, 1904 1923, 2177, 2363, 2582 2648, 2654, 2682, 2686 2727, 2731, 2785, 3068 3255, 3302, 3358, 3380 3591, 4151, 4188 Cfr10I 4 1502, 2604, 2785, 3068 ClaI 1 2124 Csp6I 11 218, 243, 298, 331 382, 539, 708, 819 892, 917, 2590 CviRI 20 668, 750, 795, 881 1033, 1060, 1128, 1845 1854, 1917, 1926, 2041 2117, 2176, 2335, 2510 2524, 3594, 3890, 4257 CviTI 79 (not expanded) DdeI 6 1765, 2067, 2948, 3397 3520, 3929 DpnI 21 586, 642, 856, 907 943, 2123, 2127, 2146 2457, 2535, 2616, 2625 2703, 3079, 3095, 3454 3466, 3544, 3552, 3563 3638 DraI 3 985, 3427, 3446 DraIII 1 1396 DrdI 3 1350, 2306, 4095 EaeI 6 655, 929, 2191, 2365 2756, 2783 EarI 3 1757, 2634, 2844 EciI 9 506, 1769, 1966, 1987 1997, 2008, 2798, 3985 4131 Ecl136II 2 564, 862 Eco52I 3 655, 929, 2191 Eco57I 3 2410, 2842, 3676 EcoO109I 1 3382 EcoRI 1 875 EcoRII 13 89, 282, 591, 782 1820, 1875, 1892, 2671 3250, 3353, 4043, 4056 4177 EheI 1 2284 FauI 14 85, 111, 278, 897 1545, 1599, 1954, 1975 2415, 2952, 3090, 3171 3237, 3303 Fnu4HI 31 654, 928, 931, 1061 1580, 1602, 1616, 2054 2193, 2245, 2256, 2346 2351, 2388, 2429, 2516 2519, 2522, 2758, 2854 2895, 2909, 3023, 3362 3377, 3588, 3794, 3797 3862, 4005, 4160 FokI 6 602, 1972, 2139, 2596 2621, 3075 FspI 1 2385 HaeII 4 1548, 1556, 2284, 3959 HaeIII 22 83, 276, 656, 900 930, 1250, 1392, 2047 2053, 2062, 2105, 2192 2366, 2757, 2784, 3293 3350, 3383, 3728, 4162 4180, 4191 HgaI 7 517, 597, 1629, 2986 3065, 3524, 4102 HhaI 20 1549, 1557, 1583, 1605 1614, 1627, 1658, 2277 2285, 2349, 2386, 2652 2912, 3088, 3174, 3577 3686, 3860, 3960, 4027 HincII 3 805, 885, 1046 HindIII 1 868 HinfI 14 410, 682, 720, 936 1328, 1350, 1760, 2770 2904, 2956, 3014, 3051 3833, 4229 HinP1I 20 1549, 1557, 1583, 1605 1614, 1627, 1658, 2277 2285, 2349, 2386, 2652 2912, 3088, 3174, 3577 3686, 3860, 3960, 4027 HpaI 1 1046 HphI 5 364, 1410, 2471, 3344 3460 KasI 1 2284 KpnI 1 891 MaeII 15 122, 134, 175, 258 339, 444, 702, 1345 1357, 1400, 1510, 2401 2588, 3369, 3502 MaeIII 13 62, 149, 498, 699 1074, 1575, 1587, 2406 2712, 3402, 3668, 3784 3847 MboII 10 627, 1529, 1757, 2634 2844, 2944, 3111, 3311 3463, 3554 MnlI 27 548, 619, 649, 849 971, 994, 1003, 1436 1767, 2045, 2051, 2058 2064, 2088, 2091, 2103 2143, 2207, 2343, 2700 2876, 3082, 3130, 3396 3766, 4033, 4090 MscI 1 2365 MslI 2 361, 2718 MspA1I 6 652, 895, 1781, 2389 3618, 3863 MspI 21 634, 658, 835, 903 913, 1503, 2190, 2267 2289, 2317, 2448, 2538 2605, 2786, 3061, 3069 3161, 3635, 3825, 3851 3998 MunI 1 1035 MvaI 13 89, 282, 591, 782 1820, 1875, 1892, 2671 3250, 3353, 4043, 4056 4177 MwoI 30 84, 206, 238, 277 370, 394, 600, 648 1506, 1536, 1563, 1605 1607, 1846, 1918, 1969 2048, 2054, 2270, 2354 2377, 2516, 2522, 2639 2722, 2989, 3176, 3380 3579, 4151 NaeI 3 1502, 2785, 3068 NarI 1 2284 NciI 9 634, 658, 902, 903 913, 2288, 2448, 3061 3825 NcoI 3 360, 2014, 2717 NdeI 1 233 NgoMIV 3 1502, 2785, 3068 NheI 1 844 NlaIII 16 301, 361, 752, 1712 1852, 1924, 2015, 2156 2501, 2687, 2718, 2744 3100, 3484, 4204, 4255 NlaIV 19 465, 636, 799, 891 899, 906, 1438, 1459 1471, 1662, 1823, 1895 2284, 2319, 3167, 3246 3291, 4134, 4173 NotI 1 928 NsiI 4 749, 1853, 1925, 4256 NspI 5 751, 1851, 1923, 2686 4203 PciI 1 4203 PleI 8 410, 720, 936, 1328 1350, 1760, 2956, 3833 Ppu10I 4 749, 1853, 1925, 4256 PsiI 2 1066, 1271 PspOMI 1 899 PstI 2 794, 880 PvuII 2 1781, 2389 RsaI 11 218, 243, 298, 331 382, 539, 708, 819 892, 917, 2590 RsrII 1 2800 SacI 2 564, 862 SacII 2 652, 895 SalI 2 805, 885 SapI 2 2634, 2844 Sau3AI 21 586, 642, 856, 907 943, 2123, 2127, 2146 2457, 2535, 2616, 2625 2703, 3079, 3095, 3454 3466, 3544, 3552, 3563 3638 Sau96I 11 83, 276, 637, 899 900, 1392, 2801, 3246 3292, 3350, 3383 SchI 8 410, 720, 936, 1328 1350, 1760, 2956, 3833 ScrFI 22 89, 282, 591, 634 658, 782, 902, 903 913, 1820, 1875, 1892 2288, 2448, 2671, 3061 3250, 3353, 3825, 4043 4056, 4177 SexAI 1 1874 SfaNI 11 367, 1092, 1855, 1927 2253, 2508, 2575, 2639 2724, 2914, 4116 SfcI 7 715, 724, 794, 880 1620, 3747, 3938 SfiI 1 2053 SmaI 1 902 SmlI 7 859, 865, 1167, 2676 3558, 3835, 4097 SnaBI 1 338 SphI 4 751, 1851, 1923, 2686 SspI 2 1191, 1744 StuI 1 2104 StyI 5 360, 2014, 2107, 2717 3122 TaiI 15 122, 134, 175, 258 339, 444, 702, 1345 1357, 1400, 1510, 2401 2588, 3369, 3502 TaqI 15 806, 860, 873, 886 1434, 2125, 2398, 2554 2578, 2614, 2776, 2967 3012, 3267, 4105 TatI 4 217, 297, 330, 381 TfiI 6 682, 2770, 2904, 3014 3051, 4229 Tru9I 16 7, 811, 986, 1047 1168, 1189, 1200, 1212 1223, 1240, 1338, 1609 3428, 3442, 3447, 3499 TseI 13 1061, 1580, 2245, 2346 2388, 2429, 2522, 2909 3362, 3588, 3794, 3797 3862 Tsp45I 4 699, 1575, 2406, 2712 Tsp509I 11 19, 876, 1036, 1100 1178, 1204, 1215, 1862 1934, 2026, 3444 TspRI 5 1125, 2408, 3518, 3789 3802 Tth111I 1 2400 VspI 1 6 XbaI 1 939 XhoI 1 859 XhoII 9 855, 906, 2456, 2702 3094, 3453, 3465, 3551 3562 XmaI 1 902 phCMV3 Restriction Sites #sites -- Bp position of recognition site -- AatII 4 121, 174, 257, 443 Acc65I 1 869 AccI 2 822, 863 AccIII 1 904 AciI 60 (not expanded) AflII 2 915, 1157 AflIII 1 4193 AluI 14 565, 841, 847, 1053 1220, 1477, 1772, 2060 2380, 2838, 3635, 3892 3938, 4028 Alw44I 1 3879 AlwI 14 642, 884, 885, 2135 2446, 2525, 2692, 3069 3084, 3443, 3456, 3541 3553, 3627 AlwNI 2 3371, 3779 ApaI 1 877 ApoI 4 853, 1089, 1193, 1204 AvaI 2 837, 880 AvaII 3 637, 2791, 3236 BamHI 1 884 BanI 6 465, 813, 869, 1428 2274, 2309 BanII 6 564, 840, 877, 1462 2636, 3346 BbeI 1 2274 BbsI 1 627 BbvI 13 1051, 1570, 2235, 2336 2378, 2419, 2512, 2899 3352, 3578, 3784, 3787 3852 BciVI 3 1693, 2483, 3984 BfaI 7 930, 1124, 1542, 2098 3113, 3447, 3700 BglI 5 84, 206, 277, 648 2044 BglII 1 833 BlnI 2 2097, 3112 BpmI 3 592, 3073, 3094 BsaAI 3 338, 1389, 2577 BsaBI 2 933, 2130 BsaHI 8 121, 174, 257, 443 597, 2274, 2976, 3055 BsaI 1 3270 BsaJI 19 360, 652, 796, 873 880, 1809, 1881, 2004 2039, 2048, 2097, 2438 2707, 3112, 3239, 3240 3342, 3343, 4033 BsaWI 5 904, 2306, 3150, 3840 3987 BseMII 6 1755, 2057, 2938, 3387 3510, 3919 BseRI 1 2078 BseSI 4 877, 2217, 2310, 3879 BsiEI 3 655, 2181, 3856 BsiHKAI 5 564, 840, 2384, 2574 3879 BslI 20 43, 652, 791, 1281 1607, 1999, 2250, 2794 3075, 3107, 3108, 3239 3274, 3324, 3325, 3356 3709, 3988, 4154, 4172 BsmAI 7 429, 595, 778, 1705 2123, 3142, 3270 BsmBI 1 595 BsmFI 11 161, 312, 480, 636 1806, 1878, 1942, 2412 2944, 3053, 3237 BsmI 2 1020, 1119 Bsp1286I 11 564, 840, 877, 1462 2217, 2310, 2384, 2574 2636, 3346, 3879 BspHI 2 1701, 3473 BspMI 3 2171, 2534, 2984 BsrBI 4 1533, 1697, 2886, 2940 BsrDI 1 2501 BsrI 8 295, 1304, 1982, 2214 2415, 3661, 3778, 3791 BssKI 21 89, 282, 591, 634 658, 796, 880, 881 1810, 1865, 1882, 2278 2438, 2661, 3051, 3240 3343, 3815, 4033, 4046 4167 BssSI 3 2867, 2993, 4020 Bst4CI 17 74, 196, 267, 541 579, 664, 785, 816 867, 1364, 2653, 2769 2877, 3200, 3691, 4161 4232 BstAPI 3 1836, 1908, 3370 BstBI 2 850, 2956 BstDSI 5 360, 652, 873, 2004 2707 BstF5I 6 602, 1962, 2129, 2586 2611, 3065 BstUI 18 59, 653, 678, 874 923, 1198, 1574, 1594 1618, 1649, 2340, 2641 3079, 3163, 3226, 3295 3568, 4149 Bsu36I 2 1754, 3386 Cac8I 29 84, 277, 810, 875 1492, 1535, 1549, 1822 1841, 1894, 1913, 2167 2353, 2572, 2638, 2644 2672, 2676, 2717, 2721 2775, 3058, 3245, 3292 3348, 3370, 3581, 4141 4178 Cfr10I 4 1492, 2594, 2775, 3058 ClaI 1 2114 Csp6I 9 218, 243, 298, 331 382, 539, 708, 870 2580 CviRI 20 668, 750, 809, 859 1023, 1050, 1118, 1835 1844, 1907, 1916, 2031 2107, 2166, 2325, 2500 2514, 3584, 3880, 4247 CviTI 78 (not expanded) DdeI 6 1755, 2057, 2938, 3387 3510, 3919 DpnI 21 586, 642, 834, 885 933, 2113, 2117, 2136 2447, 2525, 2606, 2615 2693, 3069, 3085, 3444 3456, 3534, 3542, 3553 3628 DraI 3 975, 3417, 3436 DraIII 1 1386 DrdI 3 1340, 2296, 4085 EaeI 5 655, 2181, 2355, 2746 2773 EarI 3 1747, 2624, 2834 EciI 9 506, 1759, 1956, 1977 1987, 1998, 2788, 3975 4121 Ecl136II 2 564, 840 Eco52I 2 655, 2181 Eco57I 3 2400, 2832, 3666 EcoO109I 1 3372 EcoRI 1 853 EcoRII 13 89, 282, 591, 796 1810, 1865, 1882, 2661 3240, 3343, 4033, 4046 4167 EheI 1 2274 FauI 14 85, 111, 278, 875 1535, 1589, 1944, 1965 2405, 2942, 3080, 3161 3227, 3293 Fnu4HI 30 654, 921, 1051, 1570 1592, 1606, 2044, 2183 2235, 2246, 2336, 2341 2378, 2419, 2506, 2509 2512, 2748, 2844, 2885 2899, 3013, 3352, 3367 3578, 3784, 3787, 3852 3995, 4150 FokI 6 602, 1962, 2129, 2586 2611, 3065 FspI 1 2375 HaeII 4 1538, 1546, 2274, 3949 HaeIII 22 83, 276, 656, 878 920, 1240, 1382, 2037 2043, 2052, 2095, 2182 2356, 2747, 2774, 3283 3340, 3373, 3718, 4152 4170, 4181 HgaI 7 517, 597, 1619, 2976 3055, 3514, 4092 HhaI 20 1539, 1547, 1573, 1595 1604, 1617, 1648, 2267 2275, 2339, 2376, 2642 2902, 3078, 3164, 3567 3676, 3850, 3950, 4017 HincII 3 822, 863, 1036 HindIII 1 846 HinfI 15 410, 682, 720, 765 926, 1318, 1340, 1750 2760, 2894, 2946, 3004 3041, 3823, 4219 HinP1I 20 1539, 1547, 1573, 1595 1604, 1617, 1648, 2267 2275, 2339, 2376, 2642 2902, 3078, 3164, 3567 3676, 3850, 3950, 4017 HpaI 1 1036 HphI 5 364, 1400, 2461, 3334 3450 KasI 1 2274 KpnI 1 869 MaeII 15 122, 134, 175, 258 339, 444, 702, 1335 1347, 1390, 1500, 2391 2578, 3359, 3492 MaeIII 13 62, 149, 498, 699 1064, 1565, 1577, 2396 2702, 3392, 3658, 3774 3837 MboII 10 627, 1519, 1747, 2624 2834, 2934, 3101, 3301 3453, 3544 MnlI 26 548, 619, 649, 961 984, 993, 1426, 1757 2035, 2041, 2048, 2054 2078, 2081, 2093, 2133 2197, 2333, 2690, 2866 3072, 3120, 3386, 3756 4023, 4080 MscI 1 2355 MslI 2 361, 2708 MspA1I 6 652, 873, 1771, 2379 3608, 3853 MspI 20 634, 658, 881, 905 1493, 2180, 2257, 2279 2307, 2438, 2528, 2595 2776, 3051, 3059, 3151 3625, 3815, 3841, 3988 MunI 1 1025 MvaI 13 89, 282, 591, 796 1810, 1865, 1882, 2661 3240, 3343, 4033, 4046 4167 MwoI 30 84, 206, 238, 277 370, 394, 600, 648 1496, 1526, 1553, 1595 1597, 1836, 1908, 1959 2038, 2044, 2260, 2344 2367, 2506, 2512, 2629 2712, 2979, 3166, 3370 3569, 4141 NaeI 3 1492, 2775, 3058 NarI 1 2274 NciI 8 634, 658, 880, 881 2278, 2438, 3051, 3815 NcoI 3 360, 2004, 2707 NdeI 1 233 NgoMIV 3 1492, 2775, 3058 NlaIII 16 301, 361, 752, 1702 1842, 1914, 2005, 2146 2491, 2677, 2708, 2734 3090, 3474, 4194, 4245 NlaIV 19 465, 636, 813, 869 877, 884, 1428, 1449 1461, 1652, 1813, 1885 2274, 2309, 3157, 3236 3281, 4124, 4163 NsiI 4 749, 1843, 1915, 4246 NspI 4 1841, 1913, 2676, 4193 PciI 1 4193 PleI 9 410, 720, 765, 926 1318, 1340, 1750, 2946 3823 Ppu10I 4 749, 1843, 1915, 4246 PsiI 2 1056, 1261 PspOMI 1 877 PstI 2 808, 858 PvuII 2 1771, 2379 RsaI 9 218, 243, 298, 331 382, 539, 708, 870 2580 RsrII 1 2790 SacI 2 564, 840 SacII 2 652, 873 SalI 2 822, 863 SapI 2 2624, 2834 Sau3AI 21 586, 642, 834, 885 933, 2113, 2117, 2136 2447, 2525, 2606, 2615 2693, 3069, 3085, 3444 3456, 3534, 3542, 3553 3628 Sau96I 11 83, 276, 637, 877 878, 1382, 2791, 3236 3282, 3340, 3373 SchI 9 410, 720, 765, 926 1318, 1340, 1750, 2946 3823 ScrFI 21 89, 282, 591, 634 658, 796, 880, 881 1810, 1865, 1882, 2278 2438, 2661, 3051, 3240 3343, 3815, 4033, 4046 4167 SexAI 1 1864 SfaNI 11 367, 1082, 1845, 1917 2243, 2498, 2565, 2629 2714, 2904, 4106 SfcI 8 715, 724, 771, 808 858, 1610, 3737, 3928 SfiI 1 2043 SmaI 1 880 SmlI 8 837, 843, 915, 1157 2666, 3548, 3825, 4087 SnaBI 1 338 SphI 3 1841, 1913, 2676 SspI 2 1181, 1734 StuI 1 2094 StyI 5 360, 2004, 2097, 2707 3112 TaiI 15 122, 134, 175, 258 339, 444, 702, 1335 1347, 1390, 1500, 2391 2578, 3359, 3492 TaqI 15 823, 838, 851, 864 1424, 2115, 2388, 2544 2568, 2604, 2766, 2957 3002, 3257, 4095 TatI 4 217, 297, 330, 381 TfiI 6 682, 2760, 2894, 3004 3041, 4219 Tru9I 18 7, 758, 828, 916 976, 1037, 1158, 1179 1190, 1202, 1213, 1230 1328, 1599, 3418, 3432 3437, 3489 TseI 13 1051, 1570, 2235, 2336 2378, 2419, 2512, 2899 3352, 3578, 3784, 3787 3852 Tsp45I 4 699, 1565, 2396, 2702 Tsp509I 12 19, 756, 854, 1026 1090, 1168, 1194, 1205 1852, 1924, 2016, 3434 TspRI 5 1115, 2398, 3508, 3779 3792 Tth111I 1 2390 VspI 2 6, 757 XbaI 1 929 XhoI 1 837 XhoII 9 833, 884, 2446, 2692 3084, 3443, 3455, 3541 3552 XmaI 1 880