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Neuronal Transfection
NeuroPORTER Reagent
  • High-level transfection efficiencies in neuronal cell lines, glial cells, and certain primary neurons.
  • Optimized protocols for primary neurons, neuronal cell lines, and glial cells.
  • Minimized cytotoxicity

NeuroPORTER Transfection Reagent is a unique cationic lipid specifically formulated for efficient DNA transfection into neuronal cell lines, glial cells, and certain primary neurons. Because the NeuroPORTER Transfection Reagent is formulated for DNA delivery into neuronal cells, the transfection efficiency is higher than other commercially available broad-spectrum transfection reagents. Also, compared to viral methods, transfection with the NeuroPORTER Reagent is simple and can be completed in as little as 24 hours, saving days of labor.

Contents
- Dried NeuroPORTER Reagent Lipid Film (1 vial for Cat. No. T400150 or 5 vials for Cat. No. T400750)
- Hydration Buffer (1 vial x 1.5 ml for Cat. No. T400150 or 5 vials x 1.5 ml for Cat. No. T400750)
- DNA Diluent (1 vial x 7.5 ml for Cat. # T400150 or 5 vials x 7.5 ml for Cat. No. T400750)

Storage
The NeuroPORTER Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4C upon receipt. The dried NeuroPORTER reagent is stable for 12 months and the hydrated NeuroPORTER reagent is stable for 6 months. The Hydration Buffer and DNA Diluent are stable for 12 months.


NeuroPORTER Transfection Reagent Kit
  • 1.5 ml vial, T400150 - $361
  • 5 x 1.5 ml vial, T400750 - $1,517
  • NeuroPORTER sample, 0.1 ml, T400100S - $14


NeuroPORTER™ Citations

1. Jianfeng Li, Yin Liu, and Xuming Zhang
Murine Coronavirus Induces Type I Interferon in Oligodendrocytes through Recognition by RIG-I and MDA5
J. Virol., Jul 2010; 84: 6472 - 6482.

2. Robert H. Andres, Raymond Choi, Arjun V. Pendharkar, Xavier Gaeta, Nancy Wang, Jaya K. Nathan, Joshua Y. Chua, Star W. Lee, Theo D. Palmer, Gary K. Steinberg, and Raphael Guzman
The CCR2/CCL2 Interaction Mediates the Transendothelial Recruitment of Intravascularly
Delivered Neural Stem Cells to the Ischemic Brain
Stroke, Oct 2011; 42: 2923 - 2931.


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NeuroPORTER Reagent
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NeuroPORTER Reagent

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Description
  • High-level transfection efficiencies in neuronal cell lines, glial cells, and certain primary neurons.
  • Optimized protocols for primary neurons, neuronal cell lines, and glial cells.
  • Minimized cytotoxicity

NeuroPORTER Transfection Reagent is a unique cationic lipid specifically formulated for efficient DNA transfection into neuronal cell lines, glial cells, and certain primary neurons. Because the NeuroPORTER Transfection Reagent is formulated for DNA delivery into neuronal cells, the transfection efficiency is higher than other commercially available broad-spectrum transfection reagents. Also, compared to viral methods, transfection with the NeuroPORTER Reagent is simple and can be completed in as little as 24 hours, saving days of labor.

Contents
- Dried NeuroPORTER Reagent Lipid Film (1 vial for Cat. No. T400150 or 5 vials for Cat. No. T400750)
- Hydration Buffer (1 vial x 1.5 ml for Cat. No. T400150 or 5 vials x 1.5 ml for Cat. No. T400750)
- DNA Diluent (1 vial x 7.5 ml for Cat. # T400150 or 5 vials x 7.5 ml for Cat. No. T400750)

Storage
The NeuroPORTER Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4C upon receipt. The dried NeuroPORTER reagent is stable for 12 months and the hydrated NeuroPORTER reagent is stable for 6 months. The Hydration Buffer and DNA Diluent are stable for 12 months.


NeuroPORTER Transfection Reagent Kit
  • 1.5 ml vial, T400150 - $361
  • 5 x 1.5 ml vial, T400750 - $1,517
  • NeuroPORTER sample, 0.1 ml, T400100S - $14


NeuroPORTER™ Citations

1. Jianfeng Li, Yin Liu, and Xuming Zhang
Murine Coronavirus Induces Type I Interferon in Oligodendrocytes through Recognition by RIG-I and MDA5
J. Virol., Jul 2010; 84: 6472 - 6482.

2. Robert H. Andres, Raymond Choi, Arjun V. Pendharkar, Xavier Gaeta, Nancy Wang, Jaya K. Nathan, Joshua Y. Chua, Star W. Lee, Theo D. Palmer, Gary K. Steinberg, and Raphael Guzman
The CCR2/CCL2 Interaction Mediates the Transendothelial Recruitment of Intravascularly
Delivered Neural Stem Cells to the Ischemic Brain
Stroke, Oct 2011; 42: 2923 - 2931.

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