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Neuronal Transfection
NeuroPORTER Reagent
  • High-level transfection efficiencies in neuronal cell lines, glial cells, and certain primary neurons.
  • Optimized protocols for primary neurons, neuronal cell lines, and glial cells.
  • Minimized cytotoxicity

NeuroPORTER Transfection Reagent is a unique cationic lipid specifically formulated for efficient DNA transfection into neuronal cell lines, glial cells, and certain primary neurons. Because the NeuroPORTER Transfection Reagent is formulated for DNA delivery into neuronal cells, the transfection efficiency is higher than other commercially available broad-spectrum transfection reagents. Also, compared to viral methods, transfection with the NeuroPORTER Reagent is simple and can be completed in as little as 24 hours, saving days of labor.

Contents
- Dried NeuroPORTER Reagent Lipid Film (1 vial for Cat. No. T400150 or 5 vials for Cat. No. T400750)
- Hydration Buffer (1 vial x 1.5 ml for Cat. No. T400150 or 5 vials x 1.5 ml for Cat. No. T400750)
- DNA Diluent (1 vial x 7.5 ml for Cat. # T400150 or 5 vials x 7.5 ml for Cat. No. T400750)

Storage
The NeuroPORTER Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4C upon receipt. The dried NeuroPORTER reagent is stable for 12 months and the hydrated NeuroPORTER reagent is stable for 6 months. The Hydration Buffer and DNA Diluent are stable for 12 months.


NeuroPORTER Transfection Reagent Kit
  • 1.5 ml vial, T400150
  • 5 x 1.5 ml vial, T400750
  • NeuroPORTER sample, 0.1 ml, T400100S - $14


NeuroPORTER™ Citations

A Secondary Mutation in BRAF Confers Resistance to RAF Inhibition in a BRAF V600E-Mutant Brain Tumor.
Wang, J.,et al (2018) Cancer Discov. 8(9): 113041. Link

142 triggers the generation of a retrograde signaling complex from sentinel mRNAs in axons.
Walker, C.A.,et al (2018) EMBO Reports 19: e45435. Link

Activation of PERK Elicits Memory Impairment through Inactivation of CREB and Downregulation of PSD95 After Traumatic Brain Injury.
Sen, T.,et al (2017) J. Neurosci. 37(24): 5900-5911. Link

DNA damage mediates changes in neuronal sensitivity induced by the inflammatory mediators, MCP-1 and LPS, and can be reversed by enhancing the DNA repair function of APE1.
Fehrenbacher, J.C.,et al (2017) J. Neurosci. 366(16): 23-35. Link

TLR-Independent and P2X7-Dependent Signaling Mediate Alu RNA-Induced NLRP3 Inflammasome Activation in Geographic Atrophy.
Kerur, N.,et al (2013) IOVS 54(12): 7395-7401. Link

Oligodendrocyte Regeneration after Neonatal Hypoxia Requires FoxO1-Mediated p27Kip1 Expression.
Jablonska, B.,et al (2012) J. Neurosci. 32(42): 1477514793. Link

ERK1/2 activation is a therapeutic target in age-related macular degeneration.
Dridi, S.,et al (2012) PNAS 109(34): 13781-13786. Link


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$16.00
NeuroPORTER Reagent
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NeuroPORTER Reagent

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Description
  • High-level transfection efficiencies in neuronal cell lines, glial cells, and certain primary neurons.
  • Optimized protocols for primary neurons, neuronal cell lines, and glial cells.
  • Minimized cytotoxicity

NeuroPORTER Transfection Reagent is a unique cationic lipid specifically formulated for efficient DNA transfection into neuronal cell lines, glial cells, and certain primary neurons. Because the NeuroPORTER Transfection Reagent is formulated for DNA delivery into neuronal cells, the transfection efficiency is higher than other commercially available broad-spectrum transfection reagents. Also, compared to viral methods, transfection with the NeuroPORTER Reagent is simple and can be completed in as little as 24 hours, saving days of labor.

Contents
- Dried NeuroPORTER Reagent Lipid Film (1 vial for Cat. No. T400150 or 5 vials for Cat. No. T400750)
- Hydration Buffer (1 vial x 1.5 ml for Cat. No. T400150 or 5 vials x 1.5 ml for Cat. No. T400750)
- DNA Diluent (1 vial x 7.5 ml for Cat. # T400150 or 5 vials x 7.5 ml for Cat. No. T400750)

Storage
The NeuroPORTER Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4C upon receipt. The dried NeuroPORTER reagent is stable for 12 months and the hydrated NeuroPORTER reagent is stable for 6 months. The Hydration Buffer and DNA Diluent are stable for 12 months.


NeuroPORTER Transfection Reagent Kit
  • 1.5 ml vial, T400150
  • 5 x 1.5 ml vial, T400750
  • NeuroPORTER sample, 0.1 ml, T400100S - $14


NeuroPORTER™ Citations

A Secondary Mutation in BRAF Confers Resistance to RAF Inhibition in a BRAF V600E-Mutant Brain Tumor.
Wang, J.,et al (2018) Cancer Discov. 8(9): 113041. Link

142 triggers the generation of a retrograde signaling complex from sentinel mRNAs in axons.
Walker, C.A.,et al (2018) EMBO Reports 19: e45435. Link

Activation of PERK Elicits Memory Impairment through Inactivation of CREB and Downregulation of PSD95 After Traumatic Brain Injury.
Sen, T.,et al (2017) J. Neurosci. 37(24): 5900-5911. Link

DNA damage mediates changes in neuronal sensitivity induced by the inflammatory mediators, MCP-1 and LPS, and can be reversed by enhancing the DNA repair function of APE1.
Fehrenbacher, J.C.,et al (2017) J. Neurosci. 366(16): 23-35. Link

TLR-Independent and P2X7-Dependent Signaling Mediate Alu RNA-Induced NLRP3 Inflammasome Activation in Geographic Atrophy.
Kerur, N.,et al (2013) IOVS 54(12): 7395-7401. Link

Oligodendrocyte Regeneration after Neonatal Hypoxia Requires FoxO1-Mediated p27Kip1 Expression.
Jablonska, B.,et al (2012) J. Neurosci. 32(42): 1477514793. Link

ERK1/2 activation is a therapeutic target in age-related macular degeneration.
Dridi, S.,et al (2012) PNAS 109(34): 13781-13786. Link

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