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Neuronal Transfection
NeuroFECT Reagent
  • Highest transfection efficiency for primary neurons
  • Low cytotoxicity
  • Simple and straightforward protocol

NeuroFECT™ Transfection Reagent is a novel cationic polymer created specifically for optimal transfection of primary neuronal cells. During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of NeuroFECT to biodegrade in vivo means there is essentially no cytotoxicity and therefore delivery of macromolecules into cells is maximized.

Contents
Cat. No. T800075: Hydrated NeuroFECT 1 vial x 0.75 ml
Cat. No. T800375: Hydrated NeuroFECT 5 vials x 0.75 ml

Storage
NeuroFECT Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4℃ upon receipt. When stored properly, the NeuroFECT reagent is stable for 6 months.


NeuroFECT Transfection Reagent Kit

  • 75 - 300 rxn kit, 0.75 ml, T800075 - $373
  • 375 - 1500 rxn kit, 5 x 0.75 ml, T800750 - $1,674


http://www.genlantis.comneurofect-reagent.html
$355.00
NeuroFECT Reagent
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NeuroFECT™ Transfection Reagent

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Description
Data
Citations
  • Highest transfection efficiency for primary neurons
  • Low cytotoxicity
  • Simple and straightforward protocol

NeuroFECT™ Transfection Reagent is a novel cationic polymer created specifically for optimal transfection of primary neuronal cells. During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of NeuroFECT to biodegrade in vivo means there is essentially no cytotoxicity and therefore delivery of macromolecules into cells is maximized.

Contents
Cat. No. T800075: Hydrated NeuroFECT 1 vial x 0.75 ml
Cat. No. T800375: Hydrated NeuroFECT 5 vials x 0.75 ml

Storage
NeuroFECT Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4℃ upon receipt. When stored properly, the NeuroFECT reagent is stable for 6 months.


NeuroFECT Transfection Reagent Kit

  • 75 - 300 rxn kit, 0.75 ml, T800075 - $373
  • 375 - 1500 rxn kit, 5 x 0.75 ml, T800750 - $1,674

Transfection Efficiency of NeuroFECT™ and LF2000 Reagent on Primary E18 Rat Hippocampal Cells

Figure 1: A three-day old culture of NeuroPure™ E18 Primary Rat Hippocampal Cells (6.5 x 105 cells/well in 24-well plates) were transfected with 0.5 and 1.0 μg of gWiz-GFP Expression Vector and varying ratios of NeuroFECT™ Transfection Reagent and Lipofectamine® 2000. Transfection efficiency was measure 3 days later by FACS analysis. The transfection efficiency achieved with NeuroFECT reagent; was up to 41% vs. up to 26% with Lipofectamine® 2000 reagent.

Expression of B-Galactosidase Following Transfection of Primary Rat E18 Hippocampal Cells with NeuroFECT vs. LF2000 Reagents

Figure 2: A three-day old culture of NeuroPure E18 Primary Rat Hippocampal Cells (6.5 x 105 cells/well in 24-well plates) were transfected with 0.5 and 1.0 μg of gWiz-B-galactosidase Expression Vector together with varying amounts of NeuroFECT and Lipofectamine 2000 reagent. Expression levels of B-galactosidase were up to 4.5 fold higher using the NeuroFECT™ reagent compared to Lipofectamine 2000.



Primary rat hippocampal cells were transfected with 0.5 μg and 1.0 μg of GFP expression plasmid using NeuroFECT Transfection Reagent. Forty-eight hours post-transfection, the cells were assayed for GFP expression by FACS analysis.

NeuroFECT™ Product Citations

1. Edoardo Marcora and Mary B. Kennedy
The Huntington's disease mutation impairs Huntingtin's role in the transport of NF- B from the synapse to the nucleus
Hum. Mol. Genet., Nov 2010; 19: 4373 - 4384.

2. Philip Gaughwin, Maciej Ciesla, Henry Yang, Bing Lim, and Patrik Brundin
Stage-Specific Modulation of Cortical Neuronal Development by Mmu-miR-134
Cereb Cortex, Aug 2011; 21: 1857 - 1869.

3. Caoimhín G. Concannon, Liam P. Tuffy, Petronela Weisová, Helena P. Bonner, David Dávila, Caroline Bonner, Marc C. Devocelle, Andreas Strasser, Manus W. Ward, and Jochen H.M. Prehn
AMP kinase–mediated activation of the BH3-only protein Bim couples energy depletion to stress-induced apoptosis
J. Cell Biol., Apr 2010; 189: 83 - 94.

4. Koichi Inoue, Deborah Branigan, and Zhi-Gang Xiong
Zinc-induced Neurotoxicity Mediated by Transient Receptor Potential Melastatin 7 Channels
J. Biol. Chem., Mar 2010; 285: 7430 - 7439.

5. Masahiko Watabe and Toshio Nakaki
Protein kinase CK2 regulates the formation and clearance of aggresomes in response to stress
J. Cell Sci., May 2011; 124: 1519 - 1532.

6. Elena Bevilacqua, Xinglong Wang, Mithu Majumder, Francesca Gaccioli, Celvie L. Yuan, Chuanping Wang, Xiongwei Zhu, Lindsay E. Jordan, Donalyn Scheuner, Randal J. Kaufman, Antonis E. Koromilas, Martin D. Snider, Martin Holcik, and Maria Hatzoglou
eIF2 Phosphorylation Tips the Balance to Apoptosis during Osmotic Stress
J. Biol. Chem., May 2010; 285: 17098 - 17111.

7. Sunghee Chai, Minghua Li, Deborah Branigan, Zhi-Gang Xiong, and Roger P. Simon
Activation of Acid-sensing Ion Channel 1a (ASIC1a) by Surface Trafficking
J. Biol. Chem., Apr 2010; 285: 13002 - 13011.

8. Xinglong Wang, Bo Su, Hyoung-gon Lee, Xinyi Li, George Perry, Mark A. Smith, and Xiongwei Zhu
Impaired Balance of Mitochondrial Fission and Fusion in Alzheimer's Disease
J. Neurosci., Jul 2009; 29: 9090 - 9103.

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