Neuronal Transfection
NeuroFECT Reagent
- Highest transfection efficiency for primary neurons
- Low cytotoxicity
- Simple and straightforward protocol
NeuroFECT™ Transfection Reagent is a novel cationic polymer created specifically for optimal transfection of primary neuronal cells. During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of NeuroFECT to biodegrade in vivo means there is essentially no cytotoxicity and therefore delivery of macromolecules into cells is maximized.
Contents
Cat. No. T800075: Hydrated NeuroFECT 1 vial x 0.75 ml
Cat. No. T800375: Hydrated NeuroFECT 5 vials x 0.75 ml
Storage
NeuroFECT Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4℃ upon receipt. When stored properly, the NeuroFECT reagent is stable for 6 months.
NeuroFECT Transfection Reagent Kit
- 75 - 300 rxn kit, 0.75 ml, T800075 - $355
- 375 - 1500 rxn kit, 5 x 0.75 ml, T800750 - $1,594
http://www.genlantis.comneurofect-reagent.html
$355.00
NeuroFECT™ Transfection Reagent
Description
Data
- Highest transfection efficiency for primary neurons
- Low cytotoxicity
- Simple and straightforward protocol
NeuroFECT™ Transfection Reagent is a novel cationic polymer created specifically for optimal transfection of primary neuronal cells. During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of NeuroFECT to biodegrade in vivo means there is essentially no cytotoxicity and therefore delivery of macromolecules into cells is maximized.
Contents
Cat. No. T800075: Hydrated NeuroFECT 1 vial x 0.75 ml
Cat. No. T800375: Hydrated NeuroFECT 5 vials x 0.75 ml
Storage
NeuroFECT Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4℃ upon receipt. When stored properly, the NeuroFECT reagent is stable for 6 months.
NeuroFECT Transfection Reagent Kit
- 75 - 300 rxn kit, 0.75 ml, T800075 - $355
- 375 - 1500 rxn kit, 5 x 0.75 ml, T800750 - $1,594
Transfection Efficiency of NeuroFECT™ and LF2000 Reagent on
Primary E18 Rat Hippocampal Cells
Figure 1: A three-day old culture of NeuroPure™ E18 Primary Rat Hippocampal Cells (6.5 x 105 cells/well in 24-well plates) were transfected with 0.5 and 1.0 μg of gWiz-GFP Expression Vector and varying ratios of NeuroFECT™ Transfection Reagent and Lipofectamine® 2000. Transfection efficiency was measure 3 days later by FACS analysis. The transfection efficiency achieved with NeuroFECT reagent; was up to 41% vs. up to 26% with Lipofectamine® 2000 reagent.
Expression of B-Galactosidase Following Transfection of Primary Rat E18
Hippocampal Cells with NeuroFECT vs. LF2000 Reagents
Figure 2: A three-day old culture of NeuroPure E18 Primary Rat Hippocampal Cells (6.5 x 105 cells/well in 24-well plates) were transfected with 0.5 and 1.0 μg of gWiz-B-galactosidase Expression Vector together with varying amounts of NeuroFECT and Lipofectamine 2000 reagent. Expression levels of B-galactosidase were up to 4.5 fold higher using the NeuroFECT™ reagent compared to Lipofectamine 2000.
Primary rat hippocampal cells were transfected with 0.5 μg and 1.0 μg of GFP expression plasmid using NeuroFECT Transfection Reagent. Forty-eight hours post-transfection, the cells were assayed for GFP expression by FACS analysis.
