Mycoplasma Detection
MycoScope PCR Detection Kit
- Sensitive: detection of less than 5 mycoplasma genomes per microliter of sample
- Comprehensive: all important strains of mycoplasma can be detected with the same primer set
- Simple - Fast and easy PCR reaction without the need of labeling probes
MycoScope™ PCR Mycoplasma Detection Kit utilizes PCR to detect the presence of mycoplasma contamination in cultured cells in less than three hours. The unique primers used in this kit allow detection of all important species of mycoplasma. It has been shown that 30-85% of cell cultures are infected with mycoplasma. Contaminating mycoplasma may grow slowly without killing the cells and lead to unreliable results. Testing for mycoplasma is a necessary quality control measure in most labs. Although agar cultures as well as DNA fluorochrome staining methods can be used for mycoplasma detection, polymerase chain reaction (PCR) is the established method of choice for high-sensitivity detection.
MycoScope™ PCR Mycoplasma Detection Kit utilizes PCR to detect the presence of mycoplasma contamination in cultured cells in less than three hours. The primers used in this kit anneal to the highly conserved 16S rRNA coding region in the mycoplasma genome, allowing detection of all important species of mycoplasma (see table below). Mycoplasma contaminants can be detected simply by running amplified fragments on an agarose gel. With the highly sensitive PCR assay, the MycoScope kit is capable of detecting less than 5 mycoplasma genomes per microliter of sample.
| Species Specificity: | ||
| A. laidlawii | M. agalactiae | M. arginini |
| M. arthritidis | M. bovis | M. cloacale |
| M. falconis | M. faucium | M. fermentans |
| M. hominis | M. hyorhinis | M. hyosynoviae |
| M. opalescens | M. orale | M. pneumoniae |
| M. primatum | M. pulmonis | M. salivarium |
| M. Spermatophilum | M. timone | U. urealyticum |
The MycoScope Kit has been tested for use with a variety of commercially available DNA polymerases. The kit contains a positive control DNA, and a successfully performed reaction is indicated by a distinct 500 bp band on the agarose gel. The same band also indicates the possible presence of mycoplasma species in the cell culture.
| Cat. No. | Description | Contents | Quantity |
| MY01100 | 100 Reaction kit | dNTP/Buffer Mixture (1X) | 3 x 1.4 ml |
| Primer Mix (5X) | 950 µl | ||
| Positive Control Reaction Tube | 100 µl | ||
| Molecular Biology Grade Water | 1.5 ml | ||
| MY01050 | 50 Reaction Kit | dNTP/Buffer Mixture (1X) | 2 x 1.25 ml |
| Primer Mix (5X) | 530 µl | ||
| Positive Control Reaction Tube | 50 µl | ||
| Molecular Biology Grade Water | 1.5 ml |
Storage
For best results, all reagents included in the kit should be kept frozen at -20°C. Repeated freeze-thaw cycles should be avoided.
http://www.genlantis.commycoscope-pcr-detection-kit.html
$182.00
MycoScope PCR Detection Kit
Description
- Sensitive: detection of less than 5 mycoplasma genomes per microliter of sample
- Comprehensive: all important strains of mycoplasma can be detected with the same primer set
- Simple - Fast and easy PCR reaction without the need of labeling probes
MycoScope™ PCR Mycoplasma Detection Kit utilizes PCR to detect the presence of mycoplasma contamination in cultured cells in less than three hours. The unique primers used in this kit allow detection of all important species of mycoplasma. It has been shown that 30-85% of cell cultures are infected with mycoplasma. Contaminating mycoplasma may grow slowly without killing the cells and lead to unreliable results. Testing for mycoplasma is a necessary quality control measure in most labs. Although agar cultures as well as DNA fluorochrome staining methods can be used for mycoplasma detection, polymerase chain reaction (PCR) is the established method of choice for high-sensitivity detection.
MycoScope™ PCR Mycoplasma Detection Kit utilizes PCR to detect the presence of mycoplasma contamination in cultured cells in less than three hours. The primers used in this kit anneal to the highly conserved 16S rRNA coding region in the mycoplasma genome, allowing detection of all important species of mycoplasma (see table below). Mycoplasma contaminants can be detected simply by running amplified fragments on an agarose gel. With the highly sensitive PCR assay, the MycoScope kit is capable of detecting less than 5 mycoplasma genomes per microliter of sample.
| Species Specificity: | ||
| A. laidlawii | M. agalactiae | M. arginini |
| M. arthritidis | M. bovis | M. cloacale |
| M. falconis | M. faucium | M. fermentans |
| M. hominis | M. hyorhinis | M. hyosynoviae |
| M. opalescens | M. orale | M. pneumoniae |
| M. primatum | M. pulmonis | M. salivarium |
| M. Spermatophilum | M. timone | U. urealyticum |
The MycoScope Kit has been tested for use with a variety of commercially available DNA polymerases. The kit contains a positive control DNA, and a successfully performed reaction is indicated by a distinct 500 bp band on the agarose gel. The same band also indicates the possible presence of mycoplasma species in the cell culture.
| Cat. No. | Description | Contents | Quantity |
| MY01100 | 100 Reaction kit | dNTP/Buffer Mixture (1X) | 3 x 1.4 ml |
| Primer Mix (5X) | 950 µl | ||
| Positive Control Reaction Tube | 100 µl | ||
| Molecular Biology Grade Water | 1.5 ml | ||
| MY01050 | 50 Reaction Kit | dNTP/Buffer Mixture (1X) | 2 x 1.25 ml |
| Primer Mix (5X) | 530 µl | ||
| Positive Control Reaction Tube | 50 µl | ||
| Molecular Biology Grade Water | 1.5 ml |
Storage
For best results, all reagents included in the kit should be kept frozen at -20°C. Repeated freeze-thaw cycles should be avoided.
