Home  .  Cells and Media  .  ImmunoPure NP Normal Human Cells  .  ImmunoPure PBMCs

ImmunoPure NP Normal Human Cells
ImmunoPure PBMCs
  • Proprietary reagents ensure high survival and recovery rates
  • Analyzed and characterized by Flow cytometry
  • Each lot tested to be free from infectious agents and contaminants

Peripheral blood mononuclear cells (PBMCs) are blood cells that have a round nucleus. PBMCs consist of lymphocytes, monocytes, and macrophages. These blood cells carry the duty of fighting infections as part of the immune system response. Typically about 75% of the lymphocyte population consists of T cells (CD4+ and CD8+ cells ~75%), with the other 25% being B cells and Natural Killer (NK) cells.

Because of their importance in multiple life science research areas, Genlantis is pleased to introduce the ImmunoPure NP line of peripheral blood cells. They consist of a variety of immune cells derived from the peripheral blood of normal adults, thereby qualifying them for a diverse number of cell-based experiments.

• PBMCs: these human peripheral blood cells consist of the lymphocyte and monocyte fractions of whole blood. The mononuclear cells derived from adult peripheral blood contain large numbers of mature immune cells.

ImmunoPure HD Peripheral Blood Mononuclear Cells
PBMC000 - 5.0 x 106 cells - $100
PBMC001 - 25.0 x 106 cells - $250
PBMC002 - 75.0 x 106 cells - $600

Figure 1. Figure 1: Third Party Fresh vs Thawed Viability Test

ImmunoPure NP Normal Human Cells are isolated from the peripheral blood (buffy coats) of healthy human adult donors performed at IRB-approved and FDA-licensed blood banks across the United States. The lymphocyte and monocyte fractions are isolated from whole blood using apheresis, a technology in which the blood of a donor or patient is passed through an apparatus that separates out these fractions, and returns the remainder to the donor’s circulation. After collection, the cells are treated with Ficoll® in order to deplete granulocytes, and red blood cells. These PBMCs are immediately cryopreserved by decreasing the temperature by 1 degree Celsius/minute down to -170ºC and using proprietary reagents to ensure high survival and recovery rates. ImmunoPure NP cells are high quality products and research tools that represent a diverse and heterogeneous population of donors. Genlantis ImmunoPure NP cells are backed by Genlantis’ customer satisfaction guarantee*. All cell batches are rigorously tested to be free from infectious agents and contaminants such as HIV-1, HBV, HCV, syphilis, bacterial, fungus, and mycoplasma. Our cells are routinely analyzed and characterized by Flow cytometry, using cell viability, cell purity, cell count, and CD markers (like CD34 and CD45) as indicators; this information is provided with each shipment on a lot-specific certificate of analysis. Recent independent third party testing have confirmed the high viability of the ImmunoPure NP cells (Figure 1).

ImmunoPure NP cells are shipped on dry ice. It is highly recommended that the cells are used as soon as possible after receipt in order to obtain the highest viability possible. For short term (3 weeks or less) cells should be stored at -80ºC. For longer term storage, cells should be kept in liquid nitrogen (-170ºC). Because of the nature of these cells, users should note that viability cannot be guaranteed after storage at -170ºC storage or beyond one week at -80ºC.


http://www.genlantis.comimmunopure-pbmc.html
$100.00
ImmunoPure™ PBMCs
Click to Enlarge

ImmunoPure PBMCs

Item #:immunopure-pbmcIn stockUsually ships the same business day
Price based on size, select below
ImmunoPure HD Peripheral Blood Mononuclear Cells:
Download User Manual
Quantity
| More


Description
Materials And Methods
  • Proprietary reagents ensure high survival and recovery rates
  • Analyzed and characterized by Flow cytometry
  • Each lot tested to be free from infectious agents and contaminants

Peripheral blood mononuclear cells (PBMCs) are blood cells that have a round nucleus. PBMCs consist of lymphocytes, monocytes, and macrophages. These blood cells carry the duty of fighting infections as part of the immune system response. Typically about 75% of the lymphocyte population consists of T cells (CD4+ and CD8+ cells ~75%), with the other 25% being B cells and Natural Killer (NK) cells.

Because of their importance in multiple life science research areas, Genlantis is pleased to introduce the ImmunoPure NP line of peripheral blood cells. They consist of a variety of immune cells derived from the peripheral blood of normal adults, thereby qualifying them for a diverse number of cell-based experiments.

• PBMCs: these human peripheral blood cells consist of the lymphocyte and monocyte fractions of whole blood. The mononuclear cells derived from adult peripheral blood contain large numbers of mature immune cells.



ImmunoPure HD Peripheral Blood Mononuclear Cells
PBMC000 - 5.0 x 106 cells - $100
PBMC001 - 25.0 x 106 cells - $250
PBMC002 - 75.0 x 106 cells - $600



Figure 1. Figure 1: Third Party Fresh vs Thawed Viability Test



ImmunoPure NP Normal Human Cells are isolated from the peripheral blood (buffy coats) of healthy human adult donors performed at IRB-approved and FDA-licensed blood banks across the United States. The lymphocyte and monocyte fractions are isolated from whole blood using apheresis, a technology in which the blood of a donor or patient is passed through an apparatus that separates out these fractions, and returns the remainder to the donor’s circulation. After collection, the cells are treated with Ficoll® in order to deplete granulocytes, and red blood cells. These PBMCs are immediately cryopreserved by decreasing the temperature by 1 degree Celsius/minute down to -170ºC and using proprietary reagents to ensure high survival and recovery rates. ImmunoPure NP cells are high quality products and research tools that represent a diverse and heterogeneous population of donors. Genlantis ImmunoPure NP cells are backed by Genlantis’ customer satisfaction guarantee*. All cell batches are rigorously tested to be free from infectious agents and contaminants such as HIV-1, HBV, HCV, syphilis, bacterial, fungus, and mycoplasma. Our cells are routinely analyzed and characterized by Flow cytometry, using cell viability, cell purity, cell count, and CD markers (like CD34 and CD45) as indicators; this information is provided with each shipment on a lot-specific certificate of analysis. Recent independent third party testing have confirmed the high viability of the ImmunoPure NP cells (Figure 1).



ImmunoPure NP cells are shipped on dry ice. It is highly recommended that the cells are used as soon as possible after receipt in order to obtain the highest viability possible. For short term (3 weeks or less) cells should be stored at -80ºC. For longer term storage, cells should be kept in liquid nitrogen (-170ºC). Because of the nature of these cells, users should note that viability cannot be guaranteed after storage at -170ºC storage or beyond one week at -80ºC.



A. General Medium Requirement
RPMI 1640 medium + 10% (v/v) FBS, 2 mM glutamine, 1% (v/v) nonessential amino acids, 1% (v/v) sodium pyruvate, 50 U/ml penicillin, 50 mg/ml streptomycin, and 50 mg/ml kanamycin.

B. Thawing Cells
1. Prepare a 37°C water bath.
2. Keep all samples frozen until the bath is ready.
3. Place the vials into the water bath, being careful not to submerge below the junction between the lid and the vial.
4. Place vial of cells in 37°C water bath and agitate until thawed. It is important to thaw the cells quickly; do NOT allow thawed cells to remain in freezing media any longer than necessary.
5. When only a small amount of ice remains, remove the vials and dry with a lab tissue. Clean the top of the vial with a lab tissue moistened with 70% alcohol; avoid wiping away the labeling.
6. Within about 30 sec., slowly add one milliliter of medium (containing serum) to the thawed cells.
7. Slowly add thawed cells to 8 mL of medium containing serum. Invert tube 2 or 3 times to mix or mix gently by pipetting up and down several times.
8. Centrifuge for 5 min at 400 x g.
9. Aspirate or decant the supernatant and gently resuspend the cell pellet in 10 mL of medium.
10. Remove an aliquot for cell count and proceed with experimental manipulations.
11. Culture the cells in in RPMI 1640 medium supplemented with 10% (v/v) FBS, 2 mM glutamine, 1% (v/v) nonessential amino acids, 1% (v/v) sodium pyruvate, 50 U/ml penicillin, 50 mg/ml streptomycin, and 50 mg/ml kanamycin.

NOTE: The cell suspension may form clumps after standing at room temperature. This can be avoided by preparing and using the cells promptly or by adding DNase to the suspension at a final concentration of 10 units per ml.

Stay Updated
Join our mailing list today to receive all the latest updates and information!
Corporate Headquarters
  • Genlantis, Inc.
  • 11011 Torreyana Rd
  • San Diego, CA 92121
  • 1-888-428-0558
Shop With Confidence
This shopping cart employs the highest industry strength security: 256 bit SSL E-commerce by Yahoo! We accept Visa, Master Card, American Express, Discover, PayPal
© 2013 Genlantis Inc. All rights reserved. Yahoo! Store by Solid Cactus