PrimaPure Cells
Human Fibroblasts
-
Each lot functionally characterized
-
Single donors to allow variability comparisons
-
Each lot tested negative for HIV, Hepatitis B & C, mycoplasma, bacteria, yeast, and fungi
-
16 population doublings guaranteed with Genlantis media and reagents
PrimaPure Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. All HDFs are characterized positive for expression of fibroblast surface protein.
Fibroblasts are cells that make the structural fibers and ground substance of connective tissue. Fibroblasts have a branched cytoplasm surrounding an elliptical, speckled nucleus having 1 or 2 nucleoli. Fibroblasts make collagens, glycosaminoglycans, reticular and elastic fibers, and glycoproteins found in the extracellular matrix. In growing individuals fibroblasts are dividing and synthesizing ground substance. Tissue damage stimulates fibrocytes and induces the mitosis of fibroblasts.
HDF stained for Fibroblast Surface Protein
PrimaPure Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. All HDFs are characterized positive for expression of fibroblast surface protein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.
| Cell Type | Quantity | Cell Passages | Recommended Media | Cat.
No. | |
| HDF (Adult) | >500,000 cells | 1st | Fibroblast Growth Medium | PH10605A (cells only) | |
| PH10605AK (complete system*) |
|||||
| HDF (Fetal) | >500,000 cells | 1st | Fibroblast Grwoth Medium | PH10605F
(cells only) | |
| PH10605FK (complete system*) | |||||
| HDF (Neonatal) | >500,000 cells | 1st | Fibroblast Growth Medium | PH10605N (cells only) | |
| PH10605NK (complete system*) | |||||
| * Each Complete System contains an ampoule of cryopreserved cells (> 500,000 cells), 500 ml growth medium, and a Subculture Reagent Kit (including 100 ml each of HEPES Buffered Saline Solution, trypsin/EDTA, and trypsin neutralizing solution). | |||||
Storage
Cells are cryopreserved immediately after purification and delivered on dry ice.
Place cryovials immediately in liquid nitrogen upon arrival.
Store the growth medium at 4°C in the dark.
Store the subculture reagent at -20°C upon receipt and at 4°C upon thawing.
http://www.genlantis.comhuman-fibroblasts.html
$127.00
Human Fibroblasts
Description
Manuals
-
Each lot functionally characterized
-
Single donors to allow variability comparisons
-
Each lot tested negative for HIV, Hepatitis B & C, mycoplasma, bacteria, yeast, and fungi
-
16 population doublings guaranteed with Genlantis media and reagents
PrimaPure Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. All HDFs are characterized positive for expression of fibroblast surface protein.
Fibroblasts are cells that make the structural fibers and ground substance of connective tissue. Fibroblasts have a branched cytoplasm surrounding an elliptical, speckled nucleus having 1 or 2 nucleoli. Fibroblasts make collagens, glycosaminoglycans, reticular and elastic fibers, and glycoproteins found in the extracellular matrix. In growing individuals fibroblasts are dividing and synthesizing ground substance. Tissue damage stimulates fibrocytes and induces the mitosis of fibroblasts.
HDF stained for Fibroblast Surface Protein
PrimaPure Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. All HDFs are characterized positive for expression of fibroblast surface protein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.
| Cell Type | Quantity | Cell Passages | Recommended Media | Cat.
No. | |
| HDF (Adult) | >500,000 cells | 1st | Fibroblast Growth Medium | PH10605A (cells only) | |
| PH10605AK (complete system*) |
|||||
| HDF (Fetal) | >500,000 cells | 1st | Fibroblast Grwoth Medium | PH10605F
(cells only) | |
| PH10605FK (complete system*) | |||||
| HDF (Neonatal) | >500,000 cells | 1st | Fibroblast Growth Medium | PH10605N (cells only) | |
| PH10605NK (complete system*) | |||||
| * Each Complete System contains an ampoule of cryopreserved cells (> 500,000 cells), 500 ml growth medium, and a Subculture Reagent Kit (including 100 ml each of HEPES Buffered Saline Solution, trypsin/EDTA, and trypsin neutralizing solution). | |||||
Storage
Cells are cryopreserved immediately after purification and delivered on dry ice.
Place cryovials immediately in liquid nitrogen upon arrival.
Store the growth medium at 4°C in the dark.
Store the subculture reagent at -20°C upon receipt and at 4°C upon thawing.
