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Expression Systems
gWiz™ Vectors High-Expression Vectors
  • High levels of transient gene expression
  • Suitable for in vitro and in vivo gene expression applications
  • High plasmid yield in E. coli
  • Expression ready with the following popular reporter genes: B-galactosidase, green fluorescent protein (GFP), luciferase, chloramphenicol acetyltransferase (CAT), secreted alkaline phosphatase (SEAP).

The gWiz™ Expression Vector has been systematically engineered to produce the highest levels of reporter gene expression in a wide variety of mammalian cells and tissues. The gWiz vector obtains its exceptional gene expression levels from an optimized human cytomegalovirus (CMV) promoter followed by intron A from the CMV immediate-early (IE) gene. In addition, the gWiz vector contains an optimized plasmid backbone that has been extensively altered to achieve abundant plasmid production in E. coli.

CHO-K1 cells were grown in chambered tissue slides and transfected in serum-free conditions with 1 ug of B-gal plasmid (gWiz vector or a commercial plasmid with CMV IE promoter driving B-gal = CMV-1) and 5 ul of GenePORTER® Transfection reagent. CV-1 and NIH 3T3 cells were transfected with 2 ug of plasmid DNA and 10 ul of GenePORTER reagent. After the initial 4 hours of incubation in serum-free media, 10% FBS-containing medium was added to the chambered slides. The cells were then cultured for 48 hours and then stained with X-gal according to standard procedure. Finally cells were examined with a upright microscope (Nikon E-600) equipped with a 20X pan fluor objective and DIC optics.

Contents
- gWiz Expression Vector (1 vial x 25 µg)

Storage
gWiz High Expression Vectors are shipped at -20şC.
For maximum stability, store all reagents at -20şC upon receipt.
Stable for 6 months when stored properly.



http://www.genlantis.comgwiz-vectors.html
$377.00
gWiz™ Vectors High-Expression Vectors
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gWiz™ Vectors High-Expression Vectors

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Description
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Manuals
  • High levels of transient gene expression
  • Suitable for in vitro and in vivo gene expression applications
  • High plasmid yield in E. coli
  • Expression ready with the following popular reporter genes: B-galactosidase, green fluorescent protein (GFP), luciferase, chloramphenicol acetyltransferase (CAT), secreted alkaline phosphatase (SEAP).

The gWiz™ Expression Vector has been systematically engineered to produce the highest levels of reporter gene expression in a wide variety of mammalian cells and tissues. The gWiz vector obtains its exceptional gene expression levels from an optimized human cytomegalovirus (CMV) promoter followed by intron A from the CMV immediate-early (IE) gene. In addition, the gWiz vector contains an optimized plasmid backbone that has been extensively altered to achieve abundant plasmid production in E. coli.

CHO-K1 cells were grown in chambered tissue slides and transfected in serum-free conditions with 1 ug of B-gal plasmid (gWiz vector or a commercial plasmid with CMV IE promoter driving B-gal = CMV-1) and 5 ul of GenePORTER® Transfection reagent. CV-1 and NIH 3T3 cells were transfected with 2 ug of plasmid DNA and 10 ul of GenePORTER reagent. After the initial 4 hours of incubation in serum-free media, 10% FBS-containing medium was added to the chambered slides. The cells were then cultured for 48 hours and then stained with X-gal according to standard procedure. Finally cells were examined with a upright microscope (Nikon E-600) equipped with a 20X pan fluor objective and DIC optics.

Contents
- gWiz Expression Vector (1 vial x 25 µg)

Storage
gWiz High Expression Vectors are shipped at -20şC.
For maximum stability, store all reagents at -20şC upon receipt.
Stable for 6 months when stored properly.


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