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Home  .  Transfection  .  siRNA Transfection  .  GeneSilencer® Transfection Reagent

siRNA Transfection
GeneSilencer® Transfection Reagent
  • High siRNA transfection efficiency
  • Functional gene silencing post siRNA delivery
  • Compatibility with diverse growth conditions (with and without serum)
  • Low cytotoxicity
  • Easy-to-use protocols for both adherent and suspension cells

GeneSilencer® siRNA Transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types. siRNAs are short, gene-specific double-stranded RNAs that can cause gene silencing in mammalian cells by catalytically cleaving greater than 95% of the target mRNA (1,2,3).

Contents

  • Hydrated GeneSilencer Lipid
  • siRNA Diluent (corresponding ratio for each kit)

Storage

GeneSilencer siRNA Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4ºC upon receipt. If stored properly, all components are stable for 6 months.

GeneSilencer Transfection Reagent Kit

  • 50 rxn kit, 0.18 ml, T500020 - $114
  • 200 rxn kit, 0.75 ml, T500750 - $350
  • 1000 rxn kit, 5 x 0.75 ml, T505750 - $1,475
  • 10 rxn sample kit, 0.03 ml, T500020S - $14

References

  1. Elbashir, S.M. et al. (2001) Nature 411: 494-498.
  2. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98: 9742-9747.
  3. Sharp, P.A. (2001) Genes and Development 15: 485-490.

GeneSilencer Cell Line Citations: 3T3-L1, A549, A7, AGS, ASM Transformed, B16-F1, BEL7404, Bronchial C166, C2C12, C6R, CHO-K1, DC2.4, GS-KB-3-1, H22, H441, H460, H9c2, hCMEC/D3, HCP40, HCT-116, HEK 293, HEK 293T, HEK-293, HeLa, HepG2, HL-60, HMEC-1, HPAEC, HT29, HUT-78, JAR, Jurkat, KATOIII, L/Stab-2, LNCaP, M2, McA-RH 7777, MCF-7, MDA MB-231, MDA MB-468, MDA-MB-435, MG63, MKN 28, MKN 45, MV-4-11, Neuro2a, NIH-3T3, OK, PC-12, Cortical Neurons, Hippocampal Neurons, Macrophage, Pulmonary Artery, Endothelium, Dendritic Cells(Bone Marrow), Cortical Neurons, Coronary Artery Endothelial, Peritoneal Macrophage, Dorsal Root Ganglion Neurons, Retinal Ganglion, Dorsal Root Ganglion, Schwann Cells Cardiac Fibroblast, Cardiac Myocytes (Neonatal), Lung Microvascular Endothelial Cells, Aortic Muscle Cells, Dendritic Cells, Cerebellar Neurons, Osteoblast, HMVEC, Cardiac Myocytes, T-Cells (CD3+), Cortical Neurons, Dendritic Cells, Cortical Neurons, Aveolar Type II Cells, Macrophage, Dorsal Root Ganglion Lacrimal Gland Acinar Cells, Bone Marrow, Dendritic Cells, Pulmonary Artery Endothelium, Primary HPAEC. RAW 264.7, Renca, SaOs2, Saos-2, SCCH196, SNB19, SW480, U20S


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GeneSilencer® Transfection Reagent
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GeneSilencer® Transfection Reagent

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Description
Data
siRNA Design
Citations
  • High siRNA transfection efficiency
  • Functional gene silencing post siRNA delivery
  • Compatibility with diverse growth conditions (with and without serum)
  • Low cytotoxicity
  • Easy-to-use protocols for both adherent and suspension cells

GeneSilencer® siRNA Transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types. siRNAs are short, gene-specific double-stranded RNAs that can cause gene silencing in mammalian cells by catalytically cleaving greater than 95% of the target mRNA (1,2,3).

Contents

  • Hydrated GeneSilencer Lipid
  • siRNA Diluent (corresponding ratio for each kit)

Storage

GeneSilencer siRNA Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4ºC upon receipt. If stored properly, all components are stable for 6 months.

GeneSilencer Transfection Reagent Kit

  • 50 rxn kit, 0.18 ml, T500020 - $114
  • 200 rxn kit, 0.75 ml, T500750 - $350
  • 1000 rxn kit, 5 x 0.75 ml, T505750 - $1,475
  • 10 rxn sample kit, 0.03 ml, T500020S - $14

References

  1. Elbashir, S.M. et al. (2001) Nature 411: 494-498.
  2. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98: 9742-9747.
  3. Sharp, P.A. (2001) Genes and Development 15: 485-490.

GeneSilencer Cell Line Citations: 3T3-L1, A549, A7, AGS, ASM Transformed, B16-F1, BEL7404, Bronchial C166, C2C12, C6R, CHO-K1, DC2.4, GS-KB-3-1, H22, H441, H460, H9c2, hCMEC/D3, HCP40, HCT-116, HEK 293, HEK 293T, HEK-293, HeLa, HepG2, HL-60, HMEC-1, HPAEC, HT29, HUT-78, JAR, Jurkat, KATOIII, L/Stab-2, LNCaP, M2, McA-RH 7777, MCF-7, MDA MB-231, MDA MB-468, MDA-MB-435, MG63, MKN 28, MKN 45, MV-4-11, Neuro2a, NIH-3T3, OK, PC-12, Cortical Neurons, Hippocampal Neurons, Macrophage, Pulmonary Artery, Endothelium, Dendritic Cells(Bone Marrow), Cortical Neurons, Coronary Artery Endothelial, Peritoneal Macrophage, Dorsal Root Ganglion Neurons, Retinal Ganglion, Dorsal Root Ganglion, Schwann Cells Cardiac Fibroblast, Cardiac Myocytes (Neonatal), Lung Microvascular Endothelial Cells, Aortic Muscle Cells, Dendritic Cells, Cerebellar Neurons, Osteoblast, HMVEC, Cardiac Myocytes, T-Cells (CD3+), Cortical Neurons, Dendritic Cells, Cortical Neurons, Aveolar Type II Cells, Macrophage, Dorsal Root Ganglion Lacrimal Gland Acinar Cells, Bone Marrow, Dendritic Cells, Pulmonary Artery Endothelium, Primary HPAEC. RAW 264.7, Renca, SaOs2, Saos-2, SCCH196, SNB19, SW480, U20S

GeneSilencerTM Reagent vs. siPORTTM NeoFXTM Reagent
 
HEK 293 Cells (click on images to enlarge)
HEK 293 Cells Only Negative Control
HEK 293 GeneSilencer+5nM GAPDHsiRNA HEK 293 siPORT NFX+5nM GAPDHsiRNA
HEK 293 GeneSilencer+10nM GAPDHsiRNA HEK 293 siPORT NFX+10nM GAPDHsiRNA
HEK 293 GeneSilencer+30nM GAPDHsiRNA HEK 293 siPORT NFX+30nM GAPDHsiRNA
HEK 293 GeneSilencer+50nM GAPDHsiRNA HEK 293 siPORT NFX+50nM GAPDHsiRNA

 

Figure Legend: HEK 293 cells were transfected with 5, 10, 30 or 50 nM of Silencer� FAM Labeled
GAPDH siRNA with either GeneSilencer� (Genlantis) or siPORT NeoFX (Ambion) siRNA
transfection reagents according to the manufacturer's recommended protocols. Cells were incubated for
48 hours then fixed, permeablized, and incubated with AlexaFluor� 555 phalloidin (Invitrogen), which
stains cellular actin red.  Cells were then mounted on slides and stained with DAPI (Invitrogen), which
stains the cell nuclei blue.  Transfected cells were visualized by fluorescence microscopy using identical
exposure times for FITC, TRITC, and DAPI.  The transfected GAPDH siRNA is localized in the cell cytosol
and can be seen as green fluorescent specks or dots.

 

HeLa S3 Cells (click on images to enlarge)

 

HeLa GeneSilencer+5nMGAPDH siRNA HeLa siPORT 20NFX+5nM GAPDHsiRNA
HeLa GeneSilencer+10nM GAPDHsiRNA HeLa siPORT NFX+10nM GAPDHsiRNA
HeLa GeneSilencer+30nM GAPDHsiRNA HeLa siPORT NFX+30nM GAPDHsiRNA
HeLa GeneSilencer+50nM GAPDHsiRNA HeLa siPORT NFX+50nM GAPDHsiRNA

Figure Legend: HeLa S3 cells were transfected with 5, 10, 30 or 50 nM of Silencer� FAM labeled GAPDH
siRNA with either GeneSilencer� (Genlantis) or siPORT&tm; NeoFX&tm; (Ambion) siRNA transfection
reagents according to the manufacturer's recommended protocols. Cells were incubated for 48 hours then
fixed, permeablized, and incubated with AlexaFluor� 555 phalloidin (Invitrogen), which stains cellular
actin red.  Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell
nuclei blue.  Transfected cells were visualized by fluorescence microscopy using identical exposure times for
FITC, TRITC, and DAPI.  The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as
green fluorescent specks or dots.

How to Design siRNA (Small Interfering RNA)

The sequence of the siRNA can be selected as follows:

  • 1. Start 75-100 bases downstream from the start codon �ATG� of your gene of interest.
  • 2. Locate the first �AA� dimer.
  • 3. Record the next 19 nucleotides following the AA dimer.
  • 4. Calculate the percentage of G/C content of the AA-N19 21-base sequence. It must be between 30% and 70% with 50% being ideal. If the sequence does not meet the criteria, the search continues downstream to the next �AA� dimer until this condition is met.
  • 5. The 21-base sequence is subjected to a BLAST-search (NCBI database) against EST libraries of your organism to ensure that no other gene(s) is targeted. (The complement is automatically searched as well.)
  • 6. If the conditions in either step 4 or 5 are not met, repeat steps 2 - 5.

    The sequence selection process has no other constraints. It is important to note that structure within the targeted mRNA appears to have minimal effect on the availability of the mRNA target and efficacy of the siRNA silencing approach. To-date, successful silencing has been achieved using the above method to select the target sequence, although the method is essentially random with respect to accounting for mRNA structure. Although siRNA silencing appears to be extremely effective by selecting a single target in the mRNA, it may be desirable to design and employ two independent siRNA duplexes to control for specificity of the silencing effect. This recommendation is only for specificity for it is yet unknown if the targeting of a gene by two different siRNA duplexes would be more effective than using a single siRNA duplex. It is believed that the rate-limiting component of the siRNA effect is the availability of cellular nuclease components and not mRNA target availability. Therefore, doubling the number of siRNA duplexes is not expected to double the rate or efficiency of silencing. If the selected siRNA duplex(es) do not function for silencing, the following steps are recommended. First, a search is conducted for sequencing errors in the gene and possible polymorphisms. Initial studies on the specificity of target recognition by siRNA duplexes indicates that a single point mutation located in the paired region of an siRNA duplex is sufficient to abolish target mRNA degradation. Second, a reexamination is performed to confirm whether the cell line is from the expected species. Third, a second and/or third target are selected and the corresponding siRNA duplexes prepared.

    References:
    1. Elbashir, S. M, et al. (2001a) Nature 411: 494-498.
    2. Elbashir, S. M, et al. (2001b) Genes & Dev. 15: 188-200.

  • Cell Line  Cell Type  Source  Genlantis Product Citation
    3T3-L1  Embryo  Swiss Mouse  GeneSilencer siRNA Transfection Reagent  Mizuarai, S., Miki, S., Araki, H., Takahashi, K. and Kotani, H.(2005) Identification of Dicarboxylate Carrier Slc25a10 as Malate Transporter in de Novo Fatty Acid Synthesis. J. Biol. Chem.280(37): p. 32434-32441.
    A549  Lung Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Ito, K., Yamamura, S., Essilfie-Quaye, S., Cosio, B., Ito, M., Barnes, P.J. and Adcock, I.M. (2006) Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NFκB suppression. J. Exp. Med. 203(1): 7-13.
    A549  Lung Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Stepulak, A., Sifringer, M., Rzeski, W., Endesfelder, S., Gratopp, A., Pohl, E.E., Bittigau, P., Hansen, H.H., Stryjecka-Zimmer, M., Turski, L. and Ikonomidou, C. (2005) NMDA antagonist inhibits the extracellular signal-regulated kinase pathway and suppresses cancer growth. Proc. Natl. Acad. Sci. 102: 15605 - 15610.
    A7  Melanoma  Human  GeneSilencer siRNA Transfection Reagent  Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445.
    AGS  Gastric Epithelium  Human  GeneSilencer siRNA Transfection Reagent  Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825.
    AGS  Gastric Epithelium  Human  GeneSilencer siRNA Transfection Reagent  Varro, A., Noble, P-J. M., Pritchard, D.M., Kennedy, S., Hart, C.A., Dimaline, R. and Dockray, G.J. (2004) Helicobacter pylori Induces Plasminogen Activator Inhibitor 2 in Gastric Epithelial Cells through Nuclear Factor-B and RhoA: Implications for Invasion and Apoptosis Cancer Res. 64: 1695 - 1702.
    ASM Transformed  Airway Smooth Muscle Cells  Human  GeneSilencer siRNA Transfection Reagent  Tran, T., Ens-Blackie, K., Rector, E.S., Stelmack, G.L., McNeill, K.D., Tarone, G., Gerthoffer, W.T., Unruh, H. and Halayko, A.J. (2007) Laminin-binding Integrin α7 is Required for Contractile Phenotype Expression by Human Airway Myocyte. Am. J. Respir. Cell Mol. Biol. 37(6): 668-80.
    B16-F1  Melanoma  Mouse  GeneSilencer siRNA Transfection Reagent  Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334.
    B16-F1  Melanoma  Mouse  GeneSilencer siRNA Transfection Reagent  Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664.
    BEL7404  Hepatoma  Human  GeneSilencer siRNA Transfection Reagent  Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506.
    Bronchial  Smooth Muscle  Human  GeneSilencer siRNA Transfection Reagent  Nunes, R.O., Schmidt, M., Dueck, G., Baarsma, H., Halayko, A.J., Kerstjens, H.A.M., Meurs, H. and Gosens, R. (2008) GSK-3/β-catenin signaling axis in airway smooth muscle: role in mitogenic signaling Am J Physiol Lung Cell Mol Physiol. 294: L1110 - L1118.
    C166  Yolk Sack Endothelium  Mouse  GeneSilencer siRNA Transfection Reagent  Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140.
    C2C12  Myoblast  Mouse  GeneSilencer siRNA Transfection Reagent  Evangelisti, C., Tazzari, P.L., Riccio, M., Fiume, R., Hozumi, Y., Fala, F., Goto, K.., Manzoli, L., Cocco, L. and Martelli, A.M. (2007) Nuclear diacylglycerol kinase-ζ is a negative regulator of cell cycle progression in C2C12 mouse myoblasts. FASEB J. 21: 3297 - 3307
    C6R  Glioma  Rat  GeneSilencer siRNA Transfection Reagent  Saarikangas, J., Hakanen, J., Mattila, P.K., Grumet, M., Salminen, M. and Lappalainen, P. (2008) ABBA regulates plasma-membrane and actin dynamics to promote radial glia extension. J Cell Sci. 121(Pt 9):1444-54.
    CHO-K1  Ovary  Chinese Hamster  GeneSilencer siRNA Transfection Reagent  Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ PKC-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226.
    DC2.4  Bone Marrow  Mouse  GeneSilencer siRNA Transfection Reagent  Jing, H., Yen, J-H and Ganea, D. (2004) A novel signaling pathway mediates the inhibition of CCL3/4 expression by PGE2. J. Biol. Chem. 279(53): p. 55176-55186.
    GS-KB-3-1  Epidermoid Adenocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506.
    H22  Hepatoma  Mouse  GeneSilencer siRNA Transfection Reagent  Huang, B., Lei, Z., Zhang, G-M, Li, D., Song, C., Li, B., Liu, Y., Yuan, Y., Unkeless, J., Xiong, H. and Feng, Z-H (2008) SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood 112(4): 1269-79.
    H22  Hepatoma  Mouse  GeneSilencer siRNA Transfection Reagent  Huang, B., Zhao, J., Shen, S., Li, H., He, K-L, Shen, G-X, Mayer, L., Unkeless, J., Li, D., Yuan, Y., Zhang, G.-M., Xiong, H. and Feng, Z.-H. (2007) Listeria monocytogenes Promotes Tumor Growth via Tumor Cell Toll-Like Receptor 2 Signaling. Cancer Res. 67(9): p. 4346-4352.
    H441  Lung Adenocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Zhang, Y-A., Nemunaitis, J., Samuel, S.K., Chen, P., Shen, Y. and Tong, A.W. (2006) Antitumor Activity of an Oncolytic Adenovirus-Delivered Oncogene Small Interfering RNA. Cancer Res. 66(19): 9736-9743.
    H460  Lung Cancer  Human  GeneSilencer siRNA Transfection Reagent  Ren, J., Shi, M., Liu, R., Yang, Q-H., Johnson, T., Skarnes, W.C. and Du, C. (2005) The Birc6 (Bruce) gene regulates p53 and the mitochondrial pathway of apoptosis and is essential for mouse embryonic development. Proc. Natl. Acad. Sci. USA. 102(3): p. 565-570.
    H9c2  Myoblast  Rat  GeneSilencer siRNA Transfection Reagent  Saotome, M., Safiulina, D., Szabadkai, G., Das, S., Fransson, A., Aspenstrom, P., Rizzuto, R. and Hajnoczky, G. (2008) Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase. Proc Natl Acad Sci U S A 105(52): p. 20728.
    hCMEC/D3  Brain Microvascular Endothelia  Human  GeneSilencer siRNA Transfection Reagent  Huang, W., Eum, S.Y., Andras, I.E., Hennig, B. and Toborek, M. (2009) PPAR{alpha} and PPAR{gamma} attenuate HIV-induced dysregulation of tight junction proteins by modulations of matrix metalloproteinase and proteasome activities. FASEB J 13 Jan 2009.
    HCP40  Colon adenocarcinoma  null  GeneSilencer siRNA Transfection Reagent  Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B., Balke, M., Ross, H., Johnson, S.W. and O'Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin Cancer Res. 11 (20): 7264 - 7272.
    HCT-116  Colon Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Wang, J., Rajput, A., Kan, J.L.C., Rose, R., Liu, X., Kuropatwinski, K., Hauser, J., Beko, A., Dominquez, I., Sharratt, E.A., Brattain, L., LeVea, C., Sun, F., Keane, D.M., Gibson, N.W. and Michael G. Brattain (2009) Knockdown of Ron kinase inhibits mutant PI3 kinase and reduces metastasis in human colon carcinoma. J. Biol. Chem. 284 (16) 10912-10922.
    HCT-116  Colon Adenocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O'Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Ahn, S., Nelson, C.D., Garrison, T.R., Miller, W.E. and Lefkowitz, R.J. (2003) Desensitization, internalization, and signaling functions of -arrestins demonstrated by RNA interference. Proc. Natl. Acad. Sci. 100: 1740 - 1744.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Ahn, S., Shenoy, S.K., Wei, H. and Lefkowitz, R.J. (2004) Differential Kinetic and Spatial Patterns of {beta}-Arrestin and G Protein-mediated ERK Activation by the Angiotensin II Receptor. J. Biol. Chem. 279 (34): 35518-35525.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Ahn, S., Wei, H., Garrison, T.R. and Lefkowitz, R.J. (2004) Reciprocal Regulation of Angiotensin Receptor-activated Extracellular Signal-regulated Kinases by (beta)-Arrestins 1 and 2. J. Biol. Chem. 279: 7807-7811.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Kim, J., Ahn, S., Ren, X-R., Whalen, E.J., Reiter, E., Wei, H. and Lefkowitz, R.J. (2005) Functional antagonism of different G protein-coupled receptor kinases for {beta}-arrestin-mediated angiotensin II receptor signaling. Proc. Natl. Acad. Sci.¿102: 1442 - 1447.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Kohout, T.A., Nicholas, S.L., Perry, S.J., Reinhart, G., Junger, S. and Struthers, R. (2004) Differential Desensitization, Receptor Phosphorylation, {beta}-Arrestin Recruitment, and ERK1/2 Activation by the Two Endogenous Ligands for the CC Chemokine Receptor 7. J. Biol. Chem. 279 (22): p. 23214-23222.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Liang, Y., Yu, W., Li, Y., Yan, X., Huang, Q. and Zhu, X. (2004) Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J. Cell Biol. 164 (4): 557-566.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Ren, X-R., Reiter, E., Ahn, S., Kim, J., Chen, W. and Lefkowitz, R.J. (2005) Different G protein-coupled receptor kinases govern G protein and {beta}-arrestin-mediated signaling of V2 vasopressin receptor. Proc. Natl. Acad. Sci. 102: 1448 - 1453.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Shenoy, S.K., Drake, M.T., Nelson, C.D., Houtz, D.A., Xiao, K., Madabushi, S., Reiter, E., Premont, R.T., Lichtarge, O. and Lefkowitz, R.J. (2006) {beta}-Arrestin-dependent, G Protein-independent ERK1/2 Activation by the {beta}2 Adrenergic Receptor. J. Biol. Chem. 281(2): 1261-1273.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Shikama, Y., Yamada, M., Miyashita, T. (2004) Caspase-8 and caspase-10 activate NF-B through RIP, NIK and IKK kinases. Eur. J. Immun. 33 (7): 1998-2006.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Wei, H., Ahn, S., Barnes, W.G. and Lefkowitz, R.J., (2004) Stable interaction between beta -arrestin2 and AT 1A receptor is required for beta -arrestin2 mediated activation of extracellular signal-regulated kinase 1 and 2. J. Biol. Chem. 279: 48255-48261.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787.
    HEK 293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Wu, J-H, Goswami, R., Kim, L.K., Miller, W.E., Peppel, K. and Freedman, N.J. (2005) The Platelet-derived Growth Factor Receptor-{beta} Phosphorylates and Activates G Protein-coupled Receptor Kinase-2: a Mechanism for Feedback Inhibition. J. Biol. Chem. 280(35): p. 31027-31035.
    HEK 293T  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Ahn, S., Kim, J., Hara, M.R., Ren, X. and Lefkowitz, R.J. (2009) β -arrestin2 mediates anti-apoptotic signaling through regulation of bad phosphorylation. J Biol Chem 284(13): 8855-65.
    HEK-293  Embryonic Fibroblast  Human  GeneSilencer siRNA Transfection Reagent  Barthet, G., Framery, B., Gaven, F., Pellissier, L., Reiter, E., Claeysen, S., Bockaert, J. and Dumuis, A. (2007) 5-Hydroxytryptamine 4 Receptor Activation of the Extracellular Signal-regulated Kinase Pathway Depends on Src Activation but Not on G Protein or beta-Arrestin Signaling. Mol. Biol. Cell. 18(6): 1979-1991.
    HEk-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  El-Shewy, H.M., Johnson, K.R., Lee, M-H, Jaffa, A.A., Obeid, L.M. and Luttrell, L.M. (2006) Insulin-like Growth Factors Mediate Heterotrimeric G Protein-dependent ERK1/2 Activation by Transactivating Sphingosine 1-Phosphate Receptors . J. Biol. Chem. 281(42): 31399-31407.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  El-Shewy, H.M., Lee, M-H., Obeid, L.M., Jaffa, A.A. and Luttrell, L.M. (2007) The Insulin-like Growth Factor Type 1 and Insulin-like Growth Factor Type 2/Mannose-6-phosphate Receptors Independently Regulate ERK1/2 Activity in HEK293 Cells. J. Biol. Chem. 282(36): 26150-26157.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Gesty-Palmer, D., Chen, M., Reiter, E., Ahn, S., Nelson, C.D., Wang, S., Eckhardt, A.E., Cowan, C.L., Spurney, R.F., Luttrell, L.M. and Lefkowitz, R.J. (2006) Distinct beta-Arrestin- and G Protein-dependent Pathways for Parathyroid Hormone Receptor-stimulated ERK1/2 Activation. J. Biol. Chem. 281(16): 10856-10864.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  IM Kim, DG Tilley, J Chen, NC Salazar, EJ Whalen, JD Violin, and HA Rockman (2008) Beta-blockers alprenolol and carvedilol stimulate beta-arrestin-mediated EGFR transactivation. Proc Natl Acad Sci 105(38): 14555-60.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Kani, S., Nakayama, E., Yoda, A., Onishi, N., Sougawa, N., Hazaka, Y., Umeda, T., Takeda, K., Ichijo, H., Hamada, Y. and Minami, Y. (2007) Chk2 kinase is required for methylglyoxal-induced G2/M cell-cycle checkpoint arrest: implication of cell-cycle checkpoint regulation in diabetic oxidative stress signaling. Genes Cells. 12(8): 919-928.
    HEk-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Kara, E., Crepieux, P., Gauthier, C., Martinat, N., Piketty, V., Guillou, F., and Reiter, E. (2006) A Phosphorylation Cluster of Five Serine and Threonine Residues in the C-Terminus of the Follicle-Stimulating Hormone Receptor Is Important for Desensitization But Not for β-Arrestin-Mediated ERK Activation. Mol. Endocrinol. 20(11): p. 3014-3026.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Nelson, C.D., Kovacs, J.J., Nobles, K.N., Whalen, E.J. and Lefkowitz, R.J. (2008) β-Arrestin Scaffolding of Phosphatidylinositol 4-Phosphate 5-Kinase Iα Promotes Agonist-stimulated Sequestration of the {beta}2-Adrenergic Receptor. J. Biol. Chem. 283(30): p. 21093-21101.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Noma, T., Lemaire, A., Prasad, S.V.N., Barki-Harrington, L., Tilley, D.G., Chen, J., Le Corvoisier, P., Violin, J.D., Wei, H., Lefkowitz, R.J. and Rockman, H.A. (2007) β-Arrestin mediated β1-adrenergic receptor transactivation of the EGFR confers cardioprotection. J. Clin. Invest. 117(9): p. 2445-2458.
    HEk-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Rajagopal, K., Whalen, E.J., Violin, J.D., Stiber, J.A., Rosenberg, P.B., Premont, R.T., Coffman, T.M., Rockman, H.A. and Lefkowitz, R.J. (2006) β-Arrestin2-mediated inotropic effects of the angiotensin II type 1A receptor in isolated cardiac myocytes. PNAS. 103: 16284 - 16289.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Shenoy, S.K., Xiao, K., Venkataramanan, V., Snyder, P.M., Freedman, N.J. and Weissman, A.M. (2008) NEDD4 mediates agonist-dependent ubiquitination, lysosomal targeting and degradation of the beta 2 adrenergic receptor. J. Biol. Chem. 283: 22166 - 22176.
    HEK-293  Embryonic Kidney  Human  GeneSilencer siRNA Transfection Reagent  Violin, J.D., DeWire, S.M., Barnes, W.G. and Lefkowitz, R.J. (2006) G Protein-Coupled Receptor Kinase and β-arrestin mediated desensitization of the angiotensin II type 1A receptor elucidated by diacylgylcerol dynamics. J. Biol. Chem. 281: 36411 - 36419.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Bentley, A.M., Normand, G., Hoyt, J. and King, R.W. (2007) Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis. Mol. Biol. Cell, Dec 2007; 18: 4847 - 4858.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Cassimeris, L. and Morabito, J. (2004) TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly. Mol. Biol. Cell 15 (4): 1580-1590.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Chung, J-S., Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ}PKC)-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Layzer, J.M., McCaffrey, A.P., Tanner, A.K., Huang, Zan, Kay, Mark A. and Sullenger, B.A. (2004) In vivo activity of nuclease-resistant siRNAs. RNA 10 (5): p. 766-771.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Pomerening, J.R., Ubersax, J.A. and Ferrell, J.E., Jr. (2008) Rapid Cycling and Precocious Termination of G1 Phase in Cells Expressing CDK1AF. Mol. Biol. Cell, 19: 3426 - 3441.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Shulga, N. and Pastorino, J.G. (2006) Acyl Coenzyme A-binding Protein Augments Bid-induced Mitochondrial Damage and Cell Death by Activating μ-Calpain. J. Biol. Chem. 281(41): 30824-30833.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Witherow, D.S., Garrison, T.R., Miller, W.E. and Robert J. Lefkowitz (2004) {beta}-Arrestin inhibits NF-{kappa}B activity by means of its interaction with the NF-{kappa}B inhibitor I{kappa}B{alpha}. Proc. Natl. Acad. Sci. USA 101: 8603-8607.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Yang, Q-H, Church-Hajduk, R., Ren, J., Newton, M.L. and Du, C. (2003) Omi/HtrA2 catalytic cleavage of inhibitor of apoptosis (IAP) irreversibly inactivates IAPs and facilitates caspase activity in apoptosis. Genes & Dev. 17: 1487 - 1496.
    HeLa  Cervical Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol Chem. 282 (25) 18327-18338.
    HepG2  Hepatocellular Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Pastorino, J.G. and Shulga, N. (2008) TNFalpha can provoke cleavage and activation of sterol regulatory element binding protein in ethanol exposed cells via a caspase dependent pathway that is cholesterol-insensitive. J. Biol. Chem. 283: 25638 - 25649.
    HL-60  Peripheral Blood Promyelocytic Leukemia  Human  GeneSilencer siRNA Transfection Reagent  Smirnova, I.V., Kajstura, M., Sawamura, T. and Goligorsky, M.S. (2004) Asymmetric dimethylarginine upregulates LOX-1 in activated macrophages: role in foam cell formation. AJP: Heart 287(2): p. H782-H790
    HMEC-1  Human Microvascular Endothelial Cells  Human  GeneSilencer siRNA Transfection Reagent  Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140.
    HPAEC  Pulmonary Artery Endothelium  Human  GeneSilencer siRNA Transfection Reagent  Berdyshev, E.V., Gorshkova, I., Skobeleva, A., Bittman, R., Lu, X., Dudek, S.M., Mirzapoiazova, T., Garcia, J.G.N. and Natarajan, V. (2009) FTY720 inhibits ceramide synthases and upregulates dihydrosphingosine-1-phosphate formation in human lung endothelial cells. J. Biol. Chem. 284 (9): 5467-5477.
    HT29  Colon Adenocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O'Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272.
    HUT-78  T Cells  Human  GeneSilencer siRNA Transfection Reagent  Maneechotesuwan, K., Xin, Y., Ito, K., Jazrawi, E., Lee, K-Y., Usmani, O.S., Barnes, P.J. and Adcock, I.M. (2007) Regulation of Th2 Cytokine Genes by p38 MAPK-Mediated Phosphorylation of GATA-3. J. Immunol. 178(4): 2491-2498.
    JAR  Choriocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A. and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176.
    Jurkat  T-Cell Lymphoma  Human  GeneSilencer siRNA Transfection Reagent  Nguyen, J.T. and Wells, J.A. (2003) Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc. Natl. Acad. Sci. 100: 7533  7538.
    KATOIII  Gastric Epithelium  Human  GeneSilencer siRNA Transfection Reagent  Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem: 278: 4821 - 4825.
    L/Stab-2  Fibroblast  Mouse  GeneSilencer siRNA Transfection Reagent  Park, S-Y., Kang, K-B., Thapa, N., Kim, S-Y., Lee, S-J. and Kim, I-S. (2008) Requirement of Adaptor Protein GULP during Stabilin-2-mediated Cell Corpse Engulfment. J. Biol. Chem. 283(16): 10593-10600.
    LNCaP  Prostate Cancer  Human  GeneSilencer siRNA Transfection Reagent  Takayama, K., Tsutsumi, S., Suzuki, T., Horie-Inoue, K., Ikeda, K., Kaneshiro, K. Fujimura, T., Kumagai, J., Urano, T., Sakaki, Y., Shirahige, K., Sasano, H., Takahashi, S., Kitamura, T., Ouchi, Y., Aburatani, H. and Inoue, S. (2009) Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. Cancer Res 1 69(1): p. 137.
    M2  Melanoma  Human  GeneSilencer siRNA Transfection Reagent  Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445.
    McA-RH 7777  Hepatoma  Rat  GeneSilencer siRNA Transfection Reagent  Pastorino, J.G. and Shulga, N. (2008) Tumor Necrosis Factor-{alpha} Can Provoke Cleavage and Activation of Sterol Regulatory Element-binding Protein in Ethanol-exposed Cells via a Caspase-dependent Pathway That Is Cholesterol Insensitive. J. Biol. Chem. 283: 25638 - 25649.
    MCF-7  Breast Adenocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Ikeda, K., Ogawa, S., Tsukui, T., Horie-Inoue, K., Ouchi, Y., Kato, S., Muramatsu, M. and Inoue, S. (2004) Protein Phosphatase 5 Is a Negative Regulator of Estrogen Receptor-mediated Transcription. Mol. Endocrinol. 18(5): 1131-1143.
    MDA MB-231  Breast Cancer  Human  GeneSilencer siRNA Transfection Reagent  Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem. 279(53): p. 55419-55424.
    MDA MB-468  Breast Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem.279(53): p. 55419-55424.
    MDA-MB-435  Melanoma  Human  GeneSilencer siRNA Transfection Reagent  Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A.and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176.
    MDA-MB-468  Breast Carcinoma  Human  GeneSilencer siRNA Transfection Reagent  Zoudilova, M., Kumar, P., Ge, L., Wang, P., Bokoch, G. M. and DeFea, K. A. (2007) β-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2. J. Biol. Chem. 282: 20634 - 20646.
    MG63  Osteosarcoma  Human  GeneSilencer siRNA Transfection Reagent  Ichikawa, T., Horie-Inoue, K., Ikeda, K., Blumberg, B. and Inoue, S. (2007) Vitamin K2 induces phosphorylation of protein kinase A and expression of novel target genes in osteoblastic cells. J Mol Endocrinol 1 39 (4): 239.
    MKN 28  Gastric Epithelium  Human  GeneSilencer siRNA Transfection Reagent  Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825.
    MKN 45  Gastric Epithelium  Human  GeneSilencer siRNA Transfection Reagent  Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825.
    MV-4-11  Biophenotypic B Myelomonocytic Leukemia  Human  GeneSilencer siRNA Transfection Reagent  Yang, X., Liu, L., Sternberg, D., Tang, L., Galinsky, I., DeAngelo, D. and Stone, R. (2005) The FLT3 Internal Tandem Duplication Mutation Prevents Apoptosis in Interleukin-3-Deprived BaF3 Cells Due to Protein Kinase A and Ribosomal S6 Kinase 1-Mediated BAD Phosphorylation at Serine 112. Cancer Res. 65(16): p. 7338-7347.
    Neuro2a  Neuroblastoma  Mouse  GeneSilencer siRNA Transfection Reagent  Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334.
    NIH-3T3  Fibroblast  Mouse  GeneSilencer siRNA Transfection Reagent  Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): 2324-2334.
    NIH-3T3  Fibroblast  Mouse  GeneSilencer siRNA Transfection Reagent  Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664.
    NIH-3T3  Fibroblast  Mouse  GeneSilencer siRNA Transfection Reagent  Nomachi, A., Nishita, M., Inaba, D., Enomoto, M., Hamasaki, M. and Minami, Y. (2008) Receptor Tyrosine Kinase Ror2 Mediates Wnt5a-induced Polarized Cell Migration by Activating c-Jun N-terminal Kinase via Actin-binding Protein Filamin A. J. Biol. Chem. 283(41): 27973-27981.
    OK  Kidney  Opossum  GeneSilencer siRNA Transfection Reagent  Khundmiri, S.J., Ameen, M., Delamere, N.A. and Lederer, E.D. (2008) PTH-mediated regulation of Na+ -K+ -ATPase requires Src kinase-dependent ERK Phosphorylation. Am J Physiol Renal Physiol. 295(2): F426-F437.
    OK  Kidney  Opossum  GeneSilencer siRNA Transfection Reagent  Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713.
    PC-12  Pheochromocytoma  Rat  GeneSilencer siRNA Transfection Reagent  de Barry, J., Janoshazi, A., Luc Dupont, J., Procksch, O., Chasserot-Golaz, S., Jeromin, A. and Vitale, N. (2006) Functional Implication of Neuronal Calcium Sensor-1 and Phosphoinositol 4-Kinase-β Interaction in Regulated Exocytosis of PC12 Cells. J. Biol Chem. 281(26): 18098-18111.
    Primary  Cortical Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Aarts, M., Lihara, K., Wei, W-L, Xiong, Z-G, Arundine, M., Cerwinski, W., MacDonald, J.F., Tymianski, M. (2003) A Key Role for TRPM7 Channels in Anoxic Neuronal Death. Cell 115: 863.
    Primary  Cortical Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Aleyasin, H., Cregan, S.P., Iyirhiaro, G., OHare, M.J., Callaghan, S.M., Slack, R.S. and Park, D.S. (2004) Nuclear Factor-B Modulates the p53 Response in Neurons Exposed to DNA Damage. J. Neurosci. 24 (12): 2963-2973.
    Primary  Hippocampal Neurons  Rat  GeneSilencer siRNA Transfection Reagent  Amaral, M.D. and Pozzo-Miller, L. (2007) TRPC3 Channels Are Necessary for Brain-Derived Neurotrophic Factor to Activate a Nonselective Cationic Current and to Induce Dendritic Spine Formation. J. Neurosci. 27(19): p. 5179-5189.
    Primary  Macrophage  Human  GeneSilencer siRNA Transfection Reagent  Asmis, R., Wang, Y., Xu, L., Kisgati, M., Begley, J.G. and¿Mieyal, J.J. (2005) A novel thiol oxidation-based mechanism for adriamycin-induced cell injury in human macrophages. FASEB J. published 13 September 2005, 10.1096/fj.04-2991fje.
    Primary  Pulmonary Artery Endothelium  Human  GeneSilencer siRNA Transfection Reagent  Birukova, A.A., Chatchavalvanich, S., Rios, A., Kawkitinarong, K., Garcia, J.G.N. and Birukov, K.G. (2006) Differential Regulation of Pulmonary Endothelial Monolayer Integrity by Varying Degrees of Cyclic Stretch. Am. J. Pathol. 168: 1749 - 1761.
    Primary  Cortical Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Burkhalter, J., Fiumelli, H., Erickson, J.D. and Martin, J-L. (2007) A critical role for system a amino acid transport in the regulation of dendritic development by BDNF. J Biol Chem 282: 5152 - 5159.
    Primary  Dendritic Cells (Bone Marrow)  Mouse  GeneSilencer siRNA Transfection Reagent  Chung, J-S, Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327.
    Primary  Cortical Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Cui, H., Hayashi, A., Sun, H-S, Belmares, M.P., Cobey, C., Phan, T., Schweizer, J., Salter, M.W., Wang, Y.T., Tasker, R.A., Garman, D., Rabinowitz, J., Lu, P.S. and Tymianski, M. (2007) PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors. J. Neurosci. 27(37): 9901-9915.
    Primary  Coronary Artery Endothelial  Human HCAEC  GeneSilencer siRNA Transfection Reagent  Dandapat, A., Hu, C., Sun, L. and Mehta, J.L. (2007) Small Concentrations of oxLDL Induce Capillary Tube Formation From Endothelial Cells via LOX-1 Dependent Redox-Sensitive Pathway. Arterioscler Thromb Vasc Biol.
    Primary  Peritoneal Macrophage  Mouse  GeneSilencer siRNA Transfection Reagent  de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism J. Lipid Res. 44: 674 - 685.
    Primary  Dorsal Root Ganglion Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Haruhisa Higuchi, Toshihide Yamashita, Hideki Yoshikawa, and Masaya Tohyama (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809.
    Primary  Retinal Ganglion  Rat  GeneSilencer siRNA Transfection Reagent  Hayashi, H., Campenot, R.B., Vance, D.E. and Vance, J.E. (2007) Apolipoprotein E-Containing Lipoproteins Protect Neurons from Apoptosis via a Signaling Pathway Involving Low-Density Lipoprotein Receptor-Related Protein-1. J. Neurosci. 27(8): 1933-1941.
    Primary  Dorsal Root Ganglion  Rat  GeneSilencer siRNA Transfection Reagent  Hengst, U. Cox, L.J., Macosko, E.Z. and Jaffrey, S.R. (2006) Functional and Selective RNA Interference in Developing Axons and Growth Cones. J. Neurosci. 26(21): 5727-5732.
    Primary  Schwann Cells  Mouse  GeneSilencer siRNA Transfection Reagent  Higuchi, H., Yamashita, T., Yoshikawa, H. and Tohyama, M. (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809.
    Primary  Cardiac Fibroblast  Mouse  GeneSilencer siRNA Transfection Reagent  Hu, C., Dandapat, A., Sun, L., Khan, J.A., Liu, Y., Hermonat, P.L. and Mehta, J.L. (2008) Regulation of TGFbeta 1-mediated collagen formation by LOX-1: Studies based on forced over-expression of TGFbeta 1 in wild-type and LOX-1 knockout mice cardiac fibroblasts. J. Biol. Chem. 283 (16) 10226-10231.
    Primary  Cardiac Myocytes (Neonatal)  Rat  GeneSilencer siRNA Transfection Reagent  Juhaszova, M., Zorov, D.B., Kim, S-H, Pepe, S., Fu, O., Fishbein, K.W., Ziman, B.D., Wang, S., Ytrehus, K., Antos, C.L., Olson, E.N. and Sollott, S.J. (2004) Glycogen synthase kinase-3{beta} mediates convergence of protection signaling to inhibit the mitochondrial permeability transition pore. J. Clin. Invest. 113(11): p. 1535-1549.
    Primary  Lung Microvascular Endothelial Cells  Human  GeneSilencer siRNA Transfection Reagent  Kolosova, I.A., Ma, S-F, Adyshev, D.M., Wang, P., Ohba, M., Natarajan, V., Garcia, J.G.N. and Verin, A.D. (2004) Role of CPI-17 in the regulation of endothelial cytoskeleton AJP: Lung. 287(5): L970.
    Primary  Aortic Muscle Cells  Rat  GeneSilencer siRNA Transfection Reagent  Lake, A.C. & Castellot, J.J. (2003) CCN5 modulates the antiproliferative effect of heparin and regulates cell motility in vascular smooth muscle cells. Cell Comm. & Signaling 1: 5.
    Primary  Dendritic Cells  Mouse  GeneSilencer siRNA Transfection Reagent  Li, M., Qian, H., Ichim, T.E., Ge, W-W, Popov, I.A., Rycerz, K., Neu, J., White, D., Zhong, R., Min, W-P. (2004) Induction of RNA Interference in Dendritic Cells. Immunologic Research 30 (2) 215-230.
    Primary  Dendritic Cells  Mouse  GeneSilencer siRNA Transfection Reagent  Li, M., Zhang, X., Zheng, X., Lian, D., Zhang, Z-X., Ge, W., Yang, J., Vladau, C., Suzuki, M., Chen, D., Zhong, R., Garcia, B., Jevnikar, A.M. and Min, W-P (2007) Immune Modulation and Tolerance Induction by RelB-Silenced Dendritic Cells through RNA Interference. J. Immunol. 178(9): p. 5480-5487.
    Primary  Dendritic Cells  Mouse  GeneSilencer siRNA Transfection Reagent  Liu, G., Ng, H., Akasaki, Y., Yuan, X., Ehtesham, M., Yin, D., Black , K.L. and Yu, J.S. (2004) Small interference RNA modulation of IL-10 in human monocyte-derived dendritic cells enhances the Th1 response. Eur. J. Immunol. 34: 1680-1687.
    Primary  Cerebellar Neurons  Rat  GeneSilencer siRNA Transfection Reagent  Numakawa, T., Nakayama, H., Suzuki, S., Kubo, T., Nara, F., Numakawa, Y., Yokomaku, D., Araki, T., Ishimoto, T., Ogura, A. and Taguchi, T. (2003) Nerve growth factor-induced glutamate release is via p75 receptor, ceramide and Ca2+ from ryanodine receptor in developing cerebellar neurons. J. Biol. Chem: 278: 41259-41269.
    Primary  Osteoblast  Mouse  GeneSilencer siRNA Transfection Reagent  Ohyama, Y., Nifuji, A., Maeda, Y., Amagasa, T. and Noda, M. (2004) Spaciotemporal Association and Bone Morphogenetic Protein Regulation of Sclerostin and Osterix Expression during Embryonic Osteogenesis. Endocrinology. 145(10): p. 4685-4692.
    Primary  HMVEC  Human  GeneSilencer siRNA Transfection Reagent  Radu Stefanescu, Dustin Bassett, Rozbeh Modarresi, Francisco Santiago, Mohamad Fakruddin, and Jeffrey Laurence (2008) Synergistic interactions between interferon-{gamma} and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis. Blood 112: 340 - 349.
    Primary  Cardiac Myocytes  Feline  GeneSilencer siRNA Transfection Reagent  Ramabadran, R. S., Chancey, A., Vallejo, J.G., Barger, P.M., Sivasubramanian, N. and Mann, D.L. (2008) Targeted Gene Silencing of Tumor Necrosis Factor Attenuates the Negative Inotropic Effects of Lipopolysaccharide in Isolated Contracting Cardiac Myocytes. Tex Heart Inst J. 35(1): 16-21.
    Primary  T-Cells (CD3+)  Human  GeneSilencer siRNA Transfection Reagent  Samten, B., Howard, S.T., Weis, S.E., Wu, S., Shams, H., Townsend, J.C., Safi, H. and Barnes, P.F. (2005) Cyclic AMP Response Element-Binding Protein Positively Regulates Production of IFN-{gamma} by T Cells in Response to a Microbial Pathogen. J. Immunol. 174(10): p. 6357-6363.
    Primary  Cortical Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Sato, S., Xu, J., Okuyama, S., Martinez, LB., Walsh, S.M, Jacobsen, M.T, Swan, R.J., Schlautman, J.D., Ciborowski, P. and Ikezu, T. (2008) Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J Neurosci 28(53): 14511.
    Primary  Dendritic Cells  Human  GeneSilencer siRNA Transfection Reagent  Smith, A.L., Ganesh, L., Leung, K., Jongstra-Bilen, J., Jongstra, J. and Nabel, G.J. (2007) Leukocyte-specific protein 1 interacts with DC-SIGN and mediates transport of HIV to the proteasome in dendritic cells. J. Exp. Med. 204(2): p. 421-430.
    Primary  Cortical Neurons  Mouse  GeneSilencer siRNA Transfection Reagent  Supnet, C., Grant, J., Kong, H., Westaway, D. and Mayne, M. (2006) Amyloid β-(1-42) Increases Ryanodine Receptor-3 Expression and Function in TgCRND8 Mice. J. Biol. Chem. 281: 38440 - 38447.
    Primary  Aveolar Type II Cells  Mouse  GeneSilencer siRNA Transfection Reagent  Ueno, T., Linder, S., Na, C-L, Rice, W.R., Johansson, J., and Weaver, T.E. (2004) Processing of Pulmonary Surfactant Protein B by Napsin and Cathepsin H. J. Biol. Chem. 279: 16178-16184.
    Primary  Macrophage  Mouse  GeneSilencer siRNA Transfection Reagent  Wang, Y., Chen, T., Han, C., He, D., Liu, H., An, H., Cai, Z. and Cao, X. (2007) Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Blood 110: 962 - 971.
    Primary  Dorsal Root Ganglion  Rat  GeneSilencer siRNA Transfection Reagent  Wu, K.Y., Hengst, U., Cox, L.J., Macosko, E.Z., Jeromin, A., Urquhart, E.R., and Jaffrey, S.R. (2005) Local translation of RhoA regulates growth cone collapse. Nature 436: 1020-1024.
    Primary  Lacrimal Gland Acinar Cells  Rabbit  GeneSilencer siRNA Transfection Reagent  Xie, J., Chiang, L., Contreras, J., Wu, K., Garner, J.A., Medina-Kauwe, L. and Hamm-Alvarez, S.F. (2006) Novel Fiber-Dependent Entry Mechanism for Adenovirus Serotype 5 in Lacrimal Acini. J. Virol. 80(23): 11833 - 11851.
    Primary  Bone Marrow  Mouse  GeneSilencer siRNA Transfection Reagent  Yang, R., Cai, Z., Zhang, Y., Yutzy IV, W.H., Roby, K.F. and Roden, R.B.S. (2006) CD80 in Immune Suppression by Mouse Ovarian Carcinoma¿Associated Gr-1+CD11b+ Myeloid Cells. Cancer Res 66 (13): 6807 - 6815.
    Primary  Dendritic Cells  Mouse  GeneSilencer siRNA Transfection Reagent  Zheng, X., Koropatnick, J., Li, M., Zhang, X., Ling, F., Ren, X., Hao, X., Sun, H., Vladau, C., Franek, J.A., Feng, B., Urquhart, B.L., Zhong, R., Freeman, D.J., Garcia, B. and Min, W-P. (2006) Reinstalling Antitumor Immunity by Inhibiting Tumor-Derived Immunosuppressive Molecule IDO through RNA Interference. J. Immunol. 177(8): 5639-5646.
    Primary  Pulmonary Artery Endothelium  Human  GeneSilencer siRNA Transfection Reagent  Zhuowei Li, Xhevahire Hyseni, Jacqueline D. Carter, Joleen M. Soukup, Lisa A. Dailey, and Yuh-Chin T. Huang (2006) Pollutant particles enhanced H2O2 production from NAD(P)H oxidase and mitochondria in human pulmonary artery endothelial cells. Am J Physiol Cell Physiol 291: C357 - C365.
    Primary HPAEC  Pulmonary Artery Endothelium  Human  GeneSilencer siRNA Transfection Reagent  Rentsendorj, O., Mirzapoiazova, T., Adyshev, D., Servinsky, L.E., Renne, T., Verin, A.D. and Pearse, D.B. (2008) Role of vasodilator-stimulated phosphoprotein in cGMP-mediated protection of human pulmonary artery endothelial barrier function. Am J Physiol Lung Cell Mol Physiol. 294(4): L686 -L697.
    RAW 264.7  Macrophage  Mouse  GeneSilencer siRNA Transfection Reagent  de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism. J. Lipid Res. 44: 674 - 685.
    RAW 264.7  Macrophage  Mouse  GeneSilencer siRNA Transfection Reagent  Liu, X., Yao, M., Li, N., Wang, C., Zheng, Y. and Cao, X. (2008) CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages. Blood. 112(13): 4961-4970.
    Renca  Renal Cell Carcinoma  Mouse  GeneSilencer siRNA Transfection Reagent  Ogushi, T., Takahashi, S., Takeuchi, T., Urano, T., Horie-Inoue, K., Kumagai, J., Kitamura, T., Ouchi, Y., Muramatsu, M. and Inoue, S. (2005) Estrogen Receptor-Binding Fragment-Associated Antigen 9 Is a Tumor-Promoting and Prognostic Factor for Renal Cell Carcinoma. Cancer Res. 65(9): p. 3700-3706.
    SaOs2  Osteosarcoma  Human  GeneSilencer siRNA Transfection Reagent  Chu, F.,Chou, P.M., Zheng, X., Mirkin, B.L. and Rebbaa, A.¿(2005)¿Control of Multidrug Resistance Gene mdr1 and Cancer Resistance to Chemotherapy by the Longevity Gene sirt1. Cancer Res.65 (22): p. 10183-10187.
    SaOS2  Osteosarcoma  Human  GeneSilencer siRNA Transfection Reagent  Zheng, X., Chu, F., Chou, P.M., Gallati, C., Dier, U., Mirkin, B.L., Mousa, S.A. and Rebbaa, A. (2009) Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism. Am J Physiol Cell Physiol. 296(1): C65-C74.
    Saos-2  Osteosarcoma  Human  GeneSilencer siRNA Transfection Reagent  Zheng, X., Chou, P.M., Mirkin, B.L. and Rebbaa, A. (2004) Senescence-initiated Reversal of Drug Resistance: Specific Role of Cathepsin L. Cancer Res. 64: 1773 - 1780.
    SCCH196  Sarcoma  Human  GeneSilencer siRNA Transfection Reagent  Takahashi, A., Higashino, F., Aoyagi, M., Yoshida, K., Itoh, M., Kyo, S., Ohno, T., Taira, T., Ariga, H., Nakajima, K., Hatta, M., Kobayashi, M., Sano, H., Kohgo, T. and Shindoh, M., (2003) EWS/ETS Fusions Activate Telomerase in Ewings Tumors. Cancer Res. 63: 8338-8344.
    SNB19  Glioblastoma  Human  GeneSilencer siRNA Transfection Reagent  Song, S.W., Fuller, G.N., Zheng, H. and Zhang, W. (2005) Inactivation of the Invasion Inhibitory Gene IIp45 by Alternative Splicing in Gliomas. Cancer Res. 65(9): p. 3562-3567.
    SW480  Colon Adenocarcinoma  Human  GeneSilencer siRNA Transfection Reagent  Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol. Chem. 282(25): 18327-18338.
    U20S  Osteosarcoma  Human  GeneSilencer siRNA Transfection Reagent  Hotulainen P. and Lappalainen, P. (2006) Stress fibers are generated by two distinct actin assembly mechanisms in motile cells J. Cell Biol. 173(3): 383-394.
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