Home  .  Product FAQ's  .  FAQ - NeuroPORTER Transfection Reagent

Product FAQ's
FAQ - NeuroPORTER Transfection Reagent


FAQs: NeuroPORTER™ Transfection Reagent

1. What is the NeuroPORTER Transfection Reagent?
2. Is the NeuroPORTER Transfection Reagent recommended for transfection of siRNA into primary neurons?
3. What media was used for the transfection of rat primary cortical neurons shown on the NeuroPORTER webpage?
4. What procedure is recommended for immunofluorescent antibody detection of transgenic proteins expressed in primary neurons?
5. How stable is the NeuroPORTER Transfection Reagent?

1. What is the NeuroPORTER Transfection Reagent?

The NeuroPORTER Transfection Reagent is a unique cationic lipid specifically formulated for efficient DNA transfection into neuronal cell lines, glial cells, and certain primary neurons.

[ back to top ]

2. Is the NeuroPORTER Transfection Reagent recommended for transfection of siRNA into primary neurons?

For transfection of siRNA into primary neurons, we recommend using the GeneSilencer® siRNA Transfection Reagent. Click here to see more information about GeneSilencer® siRNA Transfection Reagent.

[ back to top ]

3. What media was used for the transfection of rat primary cortical neurons shown on the NeuroPORTER webpage?

The following medium was used:
  • 500 ML DMEM/ HIGH GLUCOSE (sigma D5671)
  • 62.5 ML BOVINE CALF SERUM(defined hight and inactivate) (Hyclone)
  • 1,5 ML PEN/STREP (Sigma P-0781)
  • 6.25 ML GLUTAMINE(200 mM) (Sigma G7513)
  • 15.6 ML HEPES (Omega HB-20)
  • 62.5 ML MEDIA F-12 (Sigma F4888)
[ back to top ]

4. What procedure is recommended for immunofluorescent antibody detection of transgenic proteins expressed in primary neurons?

The following procedure was used for immunofluorescent antibody detection in primary rat cortical neurons:
  • Wash cover slips 3 times with PBS-BSA.
  • Permeabilize cells with PBS-BSA + TritonX 0.1% for 5-10 minutes at room temperature (RT).
  • Wash 1 time with PBS-BSA.
  • Add Primary Antibody diluted in PBS-BSA, TrintonX 0.05% to cells.
  • Incubate for 1 hour at RT.
  • Wash 3 times with PBS-BSA + TritonX 0.05%.
  • Add Secondary Antibody diluted in PBS-BSA, TrintonX 0.05% to cells.
  • Incubate for 1 hour at RT.
  • Wash 2 times with PBS-BSA
  • Wash 3 times with PBS.
  • Mount cover slips using media specific for fluorescence.
[ back to top ]

5. How stable is the NeuroPORTER Transfection Reagent?

All components are stable for at least one year if stored properly.

http://www.genlantis.comfaq-neuroporter.html
$1.00
FAQ - NeuroPORTER Transfection Reagent
Click to Enlarge

FAQ - NeuroPORTER Transfection Reagent

Item #:faq-neuroporterIn stockUsually ships the same business day
Price based on size, select below
Quantity
| More


Description


FAQs: NeuroPORTER™ Transfection Reagent

1. What is the NeuroPORTER Transfection Reagent?
2. Is the NeuroPORTER Transfection Reagent recommended for transfection of siRNA into primary neurons?
3. What media was used for the transfection of rat primary cortical neurons shown on the NeuroPORTER webpage?
4. What procedure is recommended for immunofluorescent antibody detection of transgenic proteins expressed in primary neurons?
5. How stable is the NeuroPORTER Transfection Reagent?

1. What is the NeuroPORTER Transfection Reagent?

The NeuroPORTER Transfection Reagent is a unique cationic lipid specifically formulated for efficient DNA transfection into neuronal cell lines, glial cells, and certain primary neurons.

[ back to top ]

2. Is the NeuroPORTER Transfection Reagent recommended for transfection of siRNA into primary neurons?

For transfection of siRNA into primary neurons, we recommend using the GeneSilencer® siRNA Transfection Reagent. Click here to see more information about GeneSilencer® siRNA Transfection Reagent.

[ back to top ]

3. What media was used for the transfection of rat primary cortical neurons shown on the NeuroPORTER webpage?

The following medium was used:
  • 500 ML DMEM/ HIGH GLUCOSE (sigma D5671)
  • 62.5 ML BOVINE CALF SERUM(defined hight and inactivate) (Hyclone)
  • 1,5 ML PEN/STREP (Sigma P-0781)
  • 6.25 ML GLUTAMINE(200 mM) (Sigma G7513)
  • 15.6 ML HEPES (Omega HB-20)
  • 62.5 ML MEDIA F-12 (Sigma F4888)
[ back to top ]

4. What procedure is recommended for immunofluorescent antibody detection of transgenic proteins expressed in primary neurons?

The following procedure was used for immunofluorescent antibody detection in primary rat cortical neurons:
  • Wash cover slips 3 times with PBS-BSA.
  • Permeabilize cells with PBS-BSA + TritonX 0.1% for 5-10 minutes at room temperature (RT).
  • Wash 1 time with PBS-BSA.
  • Add Primary Antibody diluted in PBS-BSA, TrintonX 0.05% to cells.
  • Incubate for 1 hour at RT.
  • Wash 3 times with PBS-BSA + TritonX 0.05%.
  • Add Secondary Antibody diluted in PBS-BSA, TrintonX 0.05% to cells.
  • Incubate for 1 hour at RT.
  • Wash 2 times with PBS-BSA
  • Wash 3 times with PBS.
  • Mount cover slips using media specific for fluorescence.
[ back to top ]

5. How stable is the NeuroPORTER Transfection Reagent?

All components are stable for at least one year if stored properly.
Stay Updated
Join our mailing list today to receive all the latest updates and information!
Corporate Headquarters
  • Genlantis, Inc.
  • 11011 Torreyana Rd
  • San Diego, CA 92121
  • 1-888-428-0558
Shop With Confidence
This shopping cart employs the highest industry strength security: 256 bit SSL E-commerce by Yahoo! We accept Visa, Master Card, American Express, Discover, PayPal
© 2013 Genlantis Inc. All rights reserved. Yahoo! Store by Solid Cactus