FAQ GeneSilencer® siRNA Transfection Reagent

FAQs: GeneSilencer® siRNA Transfection Reagent

1. What is the GeneSilencer® siRNA Transfection Reagent?
2. How does the GeneSilencer® siRNA Transfection Reagent work?
3. How do I design my siRNA oligonucleotide?
4. How can I determine transfection efficiency using the GeneSilencer® siRNA Transfection Reagent?

1. What is the GeneSilencer® siRNA Transfection Reagent?

The GeneSilencer® siRNA Transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types.

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2. How do I design my siRNA oligonucleotide?

Click here (pdf-80KB) to view guidelines for designing siRNA oligonucleotides.

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3. How does the GeneSilencer® siRNA Transfection Reagent work?

The GeneSilencer® siRNA Transfection Reagent works by forming non-covalent complexes with siRNA oligonucleotides, and subsequently binding to cell membranes. The siRNA/GeneSilencer complexes are taken into the cellular cytoplasm primarily via endocyctosis. Once inside the cells, the siRNA is released to achieve sequence specific gene silencing through RNA interference.

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4. How can I determine efficiency of my transfection using the GeneSilencer® siRNA Transfection Reagent?

Efficiency of siRNA transfection is commonly determined by using fluorescent-labeled siRNA oligonucleotides.