FAQ B-galactosidase kits

FAQs: β-Galactosidase and X-Gal Assay Kits

1. What β-Galactosidase and X-Gal Staining Assay Kits are available from Genlantis?
2. Can the X-gal staining kit be used to stain tissue sections?
3. What cells can I use as a positive control with your β-galactosidase assay kits?
4. What is the composition of the lysis buffer contained in the β-galactosidase kits?
5. What is the composition of the Stop Buffer contained in the β-galactosidase kits?

1. What β-Galactosidase and X-Gal Staining Assay Kits are available from Genlantis?

There are 3 kits currently available from Genlantis:

• Enhanced β-Galactosidase Assay Kit (CPRG), Cat. No. A10100K. For ultra-sensitive and quantitative measurement of low-level β-galactosidase expression.
• β-Galactosidase Assay Kit (ONPG), Cat. No. A10200K. For sensitive and quantitative measurement of β-galactosidase expression.
• X-Gal Staining Assay Kit, Cat. No. A10300K. For determination of transfection efficiency based on in situ β-galactosidase staining.

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2. Can the X-gal staining kit be used to stain tissue sections?

Yes. Follow the standard protocol. Increase the incubation time with the X-gal staining solution if necessary.

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3. What cells can I use as a positive control with your β-galactosidase assay kits?

C6 cells from ATCC, which constitutively express the lacZ gene, can be used as a positive control with our β-galactosidase assay kits.

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4. What is the composition of the lysis buffer contained in the β-galactosidase kits?

The lysis buffer composition is: 250 mM Tris, 0.1% Triton X-100, pH 7.25.

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5. What is the composition of the Stop Buffer contained in the β-galactosidase kits?

The composition of the stop buffer in the β-Galactosidase Assay Kit is: 1 M Sodium Carbonate.