The phCMV series of vectors are designed to achieve significantly higher expression levels than traditional human cytomegalovirus (CMV) promoter-based constitutive vector expression. The CMV promoter/enhancer sequence in the phCMV vectors have been systematically modified so that only the necessary sequences that confer high transcriptional activity are retained. In addition to the optimized CMV promoter, the phCMV vector includes the intron A from the human CMV immediate-early (IE) gene and an efficient artificial terminator to ensure the highest transcription levels possible. The backbones of the phCMV vectors have also been rigorously engineered to provide both high plasmid yield in E. coli and enhanced expression levels in vivo. This makes the phCMV vectors ideal tools for routine protein expression studies as well as animal injection experiments.phCMV vectors use the Kan/Neo gene for selection in both bacteria and cultured cell lines, they offer smaller vector sizes and improved transfection efficiency. Optional N- or C-terminal HA fusion tags for simplified protein detection and purification with anti-HA antibodies and affinity resins.
The gWiz high-expression vectors are a new series of plasmids that have been engineered to produce the highest levels of reporter gene expression in a wide variety of mammalian cells and tissues. You can easily monitor the efficiency of your transfections by following the expression of commonly-used reporter genes B-galactosidase, green fluorescent protein (GFP), luciferase, chloramphenicol acetyltransferase (CAT)and secreted alkaline phosphatase (SEAP).