TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.


Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null

Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax Nuclear Extraction Kit
  TM100100
 TransMax Nuclear Extraction Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null

Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax Nuclear Extraction Kit
  TM100100
 TransMax Nuclear Extraction Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NFkB Assay Kit
  TM101096
 TransMax NFkB Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null

Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax Nuclear Extraction Kit
  TM100100
 TransMax Nuclear Extraction Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NFkB Assay Kit
  TM101096
 TransMax NFkB Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NF-1 Assay Kit
  TM103096
 TransMax NF-1 Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null

Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax Nuclear Extraction Kit
  TM100100
 TransMax Nuclear Extraction Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NFkB Assay Kit
  TM101096
 TransMax NFkB Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NF-1 Assay Kit
  TM103096
 TransMax NF-1 Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax EGR Assay Kit
  TM102096
 TransMax EGR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null

Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
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  TransMax Nuclear Extraction Kit
  TM100100
 TransMax Nuclear Extraction Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NFkB Assay Kit
  TM101096
 TransMax NFkB Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NF-1 Assay Kit
  TM103096
 TransMax NF-1 Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax EGR Assay Kit
  TM102096
 TransMax EGR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax AP-1 Assay Kit
  TM105096
 TransMax AP-1 Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
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Proteomics Transcription Factor Assays

 

  TransMax Transcription Factor Assay Kits
  TMAX_01
 Chemiluminescent Kits for Transcription Factor Activation

 
  • Up to 100-fold more sensitive than gel shift assays
  • More consistent and robust results than antibody assays
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax™ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 

Description
The TransMax™ kits are chemiluminescence-based assays that enable rapid analysis of transcription factor activity in a convenient 96-well format. These sensitive, non-radioactive assays can be completed within four hours and are a superior alternative to standard gel shift assays, immunoblotting, and antibodies based ELISAs. The TransMax kits employ a proprietary assay chemistry and chemiluminescent detection and offer superior sensitivity, broad dynamic range, and excellent reproducibility compared to the standard assay approaches.

Highly Sensitive Assays Yielding Specific Results
NFκB binding activity was detected in as little as 0.004 gel shift units (GSU) of recombinant p50. (1 GSU = amount of purified NFκB protein required to completely shift 380 fmoles of NFκB binding site in a standard gel shift assay.) TransMax EGR Kit detects EGR activation in 2.5 µg of nuclear extract from Jurkat cells treated with TPA for 4 hours. Excess of unlabeled EGR binding site reduced the signal by more than 90%. Mutants binding sites predicted not to bind EGR did not reduce the specific assay signals.

The TransMax kits utilize an elegant direct quantification approach to measure the binding of activated transcription factors to their nascent DNA binding sequences. First, nuclear extracts from cells or tissues are incubated with a biotin-labeled DNA probe consisting of a double-stranded consensus transcription factor binding site and a single -stranded capture region (Figure 1). Activated transcription factors bind specifically to the double-stranded consensus sequence. Next, a double-stranded DNA specific nuclease is added to the mixture. Probes with a bound transcription factor are protected from digestion whereas unbound probes are degraded.

The reactions are then transferred to a 96-well plate coated with the complementary strand to the capture region of the probe, and the intact probe/transcription factor is captured on the plate. After unbound biotin and digested probes are washed away, the intact captured biotinylated probes are detected using a streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate. The amount of signal generated is proportional to the amount of transcription factor in the nuclear extract and can be easily detected using any standard microplate luminometer.

Detection of NFκB in small samples. NFκB activation was observed in as little as 0.1 µg of nuclear extract from TNF-a stimulated HeLa cells.

Detection of Multiple Transcription Factor Complexes. TransMax AP-1 Kit detects both c-jun homodimers and c-jun-c-fos heterdimers, obviating the need for specific antibodies to multiple protein forms or splice variants.

Contents
Each TransMax kit comes with all the reagents you need to quantitatively measure transcription factors including one PCR plate, one capture plate, two plate sealers, sample diluents, binding mixes, digestion reagents, hybridization buffer, 10X washing buffer, conjugation reagents, and chemilumiinescent substrate.

Storage
Store all components at -20ºC upon receipt. Stable for 6 months when stored proprely. Once diluted the Wash buffer may be stored at room temperature.



 

  TransMax PPAR Assay Kit
  TM104096
 TransMax PPAR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax Nuclear Extraction Kit
  TM100100
 TransMax Nuclear Extraction Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NFkB Assay Kit
  TM101096
 TransMax NFkB Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax NF-1 Assay Kit
  TM103096
 TransMax NF-1 Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax EGR Assay Kit
  TM102096
 TransMax EGR Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  TransMax AP-1 Assay Kit
  TM105096
 TransMax AP-1 Assay Kit

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
null


 

  10X Wash Buffer for TransMax Kits
  TM100200
 10X Wash Buffer for TransMax Kits

 
  • Upto 100-fold more sensitive than gel shift assays
  • Direct quantitation of binding activity without the need of antibodies
  • Minimize the amount of nuclear extract required
  • Eliminate radioactivity and the need to run gels
  • Results in less than four hours
  • Compatible iwth stndard plate luminometers
  • Stripwell format to accommodate both high and low throughput experiments

The TransMax¿ Transcription Factor Assay Kits allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells using a convenient high-throughput 96 well format. These kits offer superior sensitivity and dynamic range, excellent reproducibility and a simple, rapid protocol.



 
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