The Genlantis Custom Stable Cell Line Generation Service includes:

1. Transfection of your insert-containing plasmid into your cell line of choice.
2. Incubation and cell line recovery/growth.
3. Transfer to resistant media and incubation for selection.
4. Single foci selection/screening.
5. Stable cell line expansion.
6. Cell line and supernatant harvest & processing.
7. Western blot (with positive & negative controls).
8. Characterization and assay according to your requirements (optional)

Now, there is no need to spend your valuable time and resources on the time-consuming processes required for stable cell line generation.  Call Genlantis Technical Support to discuss your stable cell line generation requirements today.

Figure 1. gWiz/GFP Expression Vector was linearized with Sac II enzyme then transfected  into CHO-K1 cells with GenePORTER® Transfection Reagent.  Stable cells were selected with 600 mg/ml G418