Seven Fresh Neuron Types Are Now Available:
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E18 Midbrain Neurons
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E18 Hippocampal Neurons
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E18 Cortical Neurons
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P8 Cerebellar Neurons
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E18 Hypothalamus Neurons
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E18 Striatal Neurons
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E18 Spinal Cord Neurons
NeuroPure Primary Rat Neuronal Cells are live neuronal tissues isolated from micro-surgically dissected regions of Sprague/Dawley rat. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 14 days under refrigeration. NeuroPure cells are ideal for a wide variety of applications including: transfection, pharmacology studies, immunocytochemistry, and neuron development studies.
NeuroPure cells - Triple stained NeuroPure neurons after 21 days in culture. Neurofilaments-red were immunostained with rabbit anti-neurofilament antibody and goat anti-rabbit AlexaFluor 568. Glial cells-green were immunostained with a mouse anti-GFAP antibody AlexaFluor 488. DNA-blue was stained with Hoechst 33258 (bis-benzamide).
NOTE: It is highly recommended to plate the NeuroPure neurons within 1-2 days after receiving the shipment as viability of the cells may decline with time.
NeuroPure Kit Components:
| Hippocampal Neuronal Cells |
Quantity |
|
Day 18 Embryonic Sprague/Dawley Rat Hippocampal Cells |
1 vial x 2 ml
(~1 x 106 cells) |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
| Cortical Neuronal Cells |
Quantity |
|
Day 18 Embryonic Sprague/Dawley Rat Cortical Cells |
1 vial x 2 ml
(~2 x 106 cells) |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
| P8 Cerebellar Neurons |
Quantity |
|
Day 8 Post-natal Sprague/Dawley Rat Cerebellar Cells |
4 vials x 2 ml
(~4 x 107 cells) |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
| E18 Hypothalamus Neurons |
Quantity |
|
Day 18 Embryonic Sprague/Dawley Rat Hypothalamus Cells |
1 vial
1 pair of Hypothalami |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
| E18 Striatal Neurons |
Quantity |
|
Day 18 Embryonic Sprague/Dawley Rat Striatal Cells |
1 vial
1 pair of Striata |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
| E18 Spinal Cord Neurons |
Quantity |
|
Day 18 Embryonic Sprague/Dawley Rat Spinal Cord Cells |
1 vial
1 Spinal Cord |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
| E18 Midbrain Neurons |
Quantity |
|
Day 18 Embryonic Sprague/Dawley Rat Midbrain Cells |
1 vial
1 Midbrain |
|
NeuroPure Plating Medium |
1 vial x 12 ml |
| NeuroPrep Medium |
1 vial x 2.5 ml |
| NeurroPapain Enzyme |
1 vail x 5 mg |
Shipping and Storage: NeuroPure Primary Rat Neuronal Cells are shipped refrigerated. Cells are stable for up to 2 weeks when stored at 4-8 °C. However, best transfection efficiency is usually obtained within 1 week.
This product is now available for purchase worldwide. Orders placed Monday though Friday are shipped the following Wednesday for Thursday Delivery.
