Fibroblasts are cells that make the structural fibers and ground substance of connective tissue. Fibroblasts have a branched cytoplasm surrounding an elliptical, speckled nucleus having 1 or 2 nucleoli. Fibroblasts make collagens, glycosaminoglycans, reticular and elastic fibers, and glycoproteins found in the extracellular matrix. In growing individuals fibroblasts are dividing and synthesizing ground substance. Tissue damage stimulates fibrocytes and induces the mitosis of fibroblasts.

HDF stained for Fibroblast Surface Protein

PrimaPure Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. All HDFs are characterized positive for expression of fibroblast surface protein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.

Storage
Cells are cryopreserved immediately after purification and delivered on dry ice. Place cryovials immediately in liquid nitrogen upon arrival. Store the growth medium at 4°C in the dark. Store the subculture reagent at -20°C upon receipt and at 4°C upon thawing.