The keratinocyte is the major cell type of the epidermis, making up about 90% of epidermal cells. Human Epidermal Keratinocytes (HEK) perform a barrier function in the skin and mouth, protecting against invasion of bacteria and foreign particles. They also keratinize, producing a hard collagen shell. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products and for developing dermal skin substitutes. HEK has been used in numerous applications, including investigations of human epidermal development, differentiation, and cellular aspects of skin diseases.

PrimaPure HEK are primary cells isolated from normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. Cells are characterized positive for expression of Cytokeratin Peptide 14.

Storage
Cells are cryopreserved immediately after purification and delivered on dry ice. Place cryovials immediately in liquid nitrogen upon arrival. Store the growth medium at 4°C in the dark. Store the subculture reagent at -20°C upon receipt and at 4°C upon thawing.