
CellScrub™ Buffer is a unique washing buffer designed for DNA extraction and removing all complexes of DNA and cationic lipids that associate with cell surfaces during transfection. This buffer is non-toxic to cells and is able to distinguish between extracellular and intracellular plasmid DNA. When CellScrub Buffer is used as part of the cell washing procedure, more precise quantitation of the amount of DNA delivered into cells can be achieved. CellScrub Buffer improves results involving studies in DNA stability, cytometry, and gene delivery.
Contents
- CellScrub Buffer 100 ml, B100001
Storage
CellScrub Buffer should be stored at 4ºC. Stable for 6 months when stored properly.
CellScrub™ Buffer is a unique washing buffer designed for DNA extraction and removing all complexes of DNA and cationic lipids that associate with cell surfaces during transfection. This buffer is non-toxic to cells and is able to distinguish between extracellular and intracellular plasmid DNA. When CellScrub Buffer is used as part of the cell washing procedure, more precise quantitation of the amount of DNA delivered into cells can be achieved. CellScrub Buffer improves results involving studies in DNA stability, cytometry, and gene delivery.
Contents
- CellScrub Buffer 100 ml, B100001
Storage
CellScrub Buffer should be stored at 4ºC. Stable for 6 months when stored properly.
Removal of Extracellular Cationic Lipid/DNA Complexes Using CellScrub™ Buffer
Sample Protocol
Culture adherent cells in 6-well plates and transfect with 5 μg of DNA complexed with cationic lipids per well as described.