CellScrub Buffer

• Effective removal of extracellular lipid/DNA complexes post-transfection
• Non-toxic to cells
• Fast and easy procedure

CellScrub™ Buffer is a unique washing buffer designed for DNA extraction and removing all complexes of DNA and cationic lipids that associate with cell surfaces during transfection. This buffer is non-toxic to cells and is able to distinguish between extracellular and intracellular plasmid DNA. When CellScrub Buffer is used as part of the cell washing procedure, more precise quantitation of the amount of DNA delivered into cells can be achieved.  CellScrub Buffer improves results involving studies in DNA stability, cytometry, and gene delivery.

Contents
- CellScrub Buffer 100 ml, B100001

Storage
CellScrub Buffer should be stored at 4ºC.  Stable for 6 months when stored properly.

Removal of Extracellular Cationic Lipid/DNA Complexes Using CellScrub™ Buffer

Adherent cells were incubated with cationic lipid/DNA complexes at 4°C for 1-2 hours. Several washes removed cell-associated complexes. After washes, cells were lysed, and the DNA was extracted. DNA samples were analyzed by agarose gel electrophoresis. DNA was stained with ethidium bromide. Arrows show plasmid DNA.
Lane 1: Trypsinization and PBS washes.
Lane 2: Washing procedure including the CellScrub™ washing buffer.

Sample Protocol

Culture adherent cells in 6-well plates and transfect with 5 μg of DNA complexed with cationic lipids per well as described.
Remove culture medium and wash cells with PBS (containing CaCl2 and MgCl2).
Treat cells with CellScrub buffer (1-2 ml/well) and incubate at room temperature for 10 to 15 minutes to remove extracellular cationic lipid/DNA complexes.
Wash twice with PBS (without CaCl2 and MgCl2).