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Cell Lysis

Cell Lysis

Lysis refers to the breaking down of the cell, often by viral, enzymic, or osmotic mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a "lysate". Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA. Lysis is used in western and Southern blotting to analyze specific proteins, lipids, nucleic acids, reporter assays, immunoassays, and protein purification. Depending upon the detergent used, either all or some membranes are lysed. If the cell membrane only is lysed then gradient centrifugation can be used for collecting organelles.

SoluLyse-M™ Mammalian Protein Extraction Reagent is a novel nonionic detergent designed for efficient whole-cell protein extraction from cultured mammalian cells. It gently and rapidly dissolves cell membranes at low concentrations without denaturing proteins.

SoluLyse™ Protein Extraction Reagent is a proprietary formulation of nonionic detergents that is optimized for the most efficient extraction of soluble proteins from bacterial cells. It allows perforation of bacterial cell wall without denaturing proteins, and there is no need for secondary treatment such as sonication or freeze-thaw.

"In our comparison study, SoluLyse reagent has shown to be the method with the highest correlation to sonication. Interestingly, widely used lysozyme and Bugbuster® reagent failed in most our cases to completely release the soluble protein. When sonication is inconvenient, or difficult to apply to many samples, we find SoluLyse® reagent to be an acceptable alternative for the ACP fragment proteins as well as many other constructs we have examined to date.”
Link http://pubmedcentralcanada.ca/pmcc/articles/PMC2855807/pdf/10969_2009_Article_9077.pdf
Authors: Pawel Listwan, Jean-Denis Pédelacq, Meghan Lockard, Carolyn Bell,1 Thomas C. Terwilliger, and Geoffrey S. Waldo J Struct Funct Genomics 2010 March; 11(1): 41–49.


SoluLyse-M Protein Extraction Reagent
SoluLyse Reagent for Bacteria
Efficient extraction of proteins from mammalian cells
Efficient and gentle extraction of soluble proteins
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