Description
The TAP Express® Rapid Gene Expression System is a novel method for high-throughput gene expression that eliminates the need for cloning by adding a strong eukaryotic promoter and terminator sequence to any gene of interest via a simple 2-step PCR procedure. The resulting linear TAP Express fragment is transcriptionally active and can be expressed in vitro or in vivo. Because the TAP Express® System eliminates the need for cloning, bacterial transformations, and plasmid purifications, large numbers of genes can be shotgun expressed and examined simultaneously. The levels of gene expression obtained with the TAP Express System are identical to those typically observed with conventional plasmid vectors, but with the TAP Express System these results are obtained much faster.
How TAP Express System Works
Contents
-TAP Express® Promoter Mix (1 vial x 40 µl for TAP010210 or
1 vial x 200 µl for TAP010110)
-TAP Express® Terminator Mix A (1 vial x 40 µl for TAP010210 or 1
vial x 200 µl for TAP010110)
-TAP Express® Terminator Mix B (1 vial x 40 µl for TAP010210 or 1
vial x 200 µl for TAP010110)
-TAP Control 5' Primer & TAP Control 3' Primer (1 vial x 10 µl for each
primer)
-TAP Control DNA Template GFP Gene Positive Control Template (1 vial x 10 µl)
-GenePORTER® Transfection Reagent (1 vial x 100 µl)
Storage
The TAP Express® rapid gene expression kit is shipped frozen. For maximum stability and long-term use, store all reagent tubes at -20ºC upon receipt. Stable for 6 months when stored properly.
US Patent 6,280,977
