The BaculoPORTER™ Transfection Reagent is a lipid formulation of a novel proprietary cationic lipid and dioleoyl phosphatidylethanolamine (DOPE) in a ratio targeted and optimized for the delivery of DNA into insect cells, especially Sf9 and Sf21.
Description The BaculoPORTER™ Transfection Reagent is a lipid formulation of a novel proprietary cationic lipid and dioleoyl phosphatidylethanolamine (DOPE) in a ratio targeted and optimized for the delivery of DNA into insect cells, especially Sf9 and Sf21. When mixed with DNA, the BaculoPORTER Transfection Reagent coats and interacts with the DNA to form a lipoplex of lipid and DNA. The formed lipoplex is efficient at delivering the target DNA into insect cells and with minimal toxicity. The BaculoPORTER Transfection Reagent allows researchers to maximize the delivery of DNA combinations including, plasmid, transfer vector and baculovirus DNA, or Bacmid DNA into insect cells to optimize expression and maximize viral titer production. When compared to other commercially available transfection reagents, The BaculoPORTER™ Transfection Reagent consistently offers superior transfection efficiencies and viral titer production.
Sf21 Cell Transfection: BaculoPORTER™ vs. Cellfectin®
Tissue Culture Dish
Cell Seeding Density per Well or Plate
DNA Amount to Use per Well or Plate (mg)
BaculoPORTER Amount to Use per Well or Plate(ml)
Final Transfection Volume per Well or Plate
96 well
15,000
1.0
100 ml
24 well
60,000
4.0
250 ml
6 well OR
35 mm
240,000
16.0
1 ml
60 mm
400,000
25.0
2.5 ml
100 mm
700,000
45.0
5 ml
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