Description
Antisense oligonucleotides, delivered into cells with cationic lipids, are standard tools for the elucidation of gene function (1,2). However, there are two main problems associated with lipid-mediated delivery of antisense oligonucleotides: poor transfection efficiency and/or high levels of cytotoxicity. Cytofectin™ Transfection Reagent alleviates both of these problems, delivering oligonucleotides efficiently into a broad spectrum of cell lines. Cytofectin Transfection Reagent corresponds to Gilead Sciences' GS 3815 which is a more stable formulation of GS 2888, first reported by Lewis, et al. (3).

Contents
Cytofectin™ Reagent Lipid (1 x 1 ml for Cat. No. T610001 or 5 x 1 ml for Cat. No. T610005) 

Storage
The Cytofectin™ Transfection Reagent is shipped on ice. Store all reagents at 4ºC upon receipt. If stored properly, all components are stable for 3 months. For longer term stability store at -70ºC.

References:
1. Wagner, RW et al. (1994) Nature 372: 333-335.
2. Wagner, RW et al. (1993) Science 260: 1510-1513.
3. Lewis, JG et al (1996) Proc. Natl. Acad. U.S.A. 93: 3176-3181.
4. Axel, DI et al. (2000) J Vasc Res. 37(4): 221-234.