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Reporter Vectors and Assay Kits
-Gal Assay Kits
  • Quantitative, sensitive, and convenient assays for determining -galactosidase expression
  • Complete kits with ready-to-use reagents, including the E. coli -galactosidase enzyme standard
  • Suitable for direct transfection assays in 96-well plates
  • Elimination of freeze-thaw cycles

The -Galactosidase Assay Kits quantitatively measure the expression level of -galactosidase in cells transfected with vectors carrying the lacZ gene. Two -Galactosidase Assay Kits are available: the highly sensitive Enhanced -Galactosidase Assay Kit (CPRG) Assay Kit, which uses the red substrate chlorophenol red--D-galactopyranoside (CPRG) to determine -gal expression levels, and the -Galactosidase Assay Kit (ONPG) Assay Kit, which measures development of the bright yellow substrate o-nitrophenyl--D-galactopyranoside (ONPG). The ONPG kit provides a quick and sensitive method for quantifying -galactosidase expression in cultured cells or tissues, while the CPRG kit is ideal for conditions in which β-galactosidse expression levels are low.

Contents
The -Galactosidase Assay Kits provide all the required reagents for 500 microassays in a 96-well plate and can be easily adapted for different assay sizes.
– 5X Lysis Buffer (1 vial x 55 ml)
– Standard Dilution Buffer (1 vial x 55 ml)
– ONPG Substrate Solution, 3mg/ml (o-nitrophenyl--D-galactopyranoside) (1 vial x 55 ml) OR 10X CPRG Substrate Stock (Chlorophenol red--D-galactopyranoside) (5 vials x 1 ml)
– Substrate Buffer (1 vial x 55 ml)
– Stop Buffer (1 vial x 55 ml)
– -galactosidase enzyme standard, 40 units (1 vial x 100 ml)

Shipping and Storage
Both -Galactosidase Assay Kits are shipped frozen.
The CPRG substrate solutions and -galactosidase enzyme standard should be stored at -20C.
All other components should be stored at 4C.
Stable for 6 months when stored properly.


http://www.genlantis.comb-gal-assay-kits.html
$247.00
-Gal Assay Kits
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-Gal Assay Kits

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Description
Data
  • Quantitative, sensitive, and convenient assays for determining -galactosidase expression
  • Complete kits with ready-to-use reagents, including the E. coli -galactosidase enzyme standard
  • Suitable for direct transfection assays in 96-well plates
  • Elimination of freeze-thaw cycles

The -Galactosidase Assay Kits quantitatively measure the expression level of -galactosidase in cells transfected with vectors carrying the lacZ gene. Two -Galactosidase Assay Kits are available: the highly sensitive Enhanced -Galactosidase Assay Kit (CPRG) Assay Kit, which uses the red substrate chlorophenol red--D-galactopyranoside (CPRG) to determine -gal expression levels, and the -Galactosidase Assay Kit (ONPG) Assay Kit, which measures development of the bright yellow substrate o-nitrophenyl--D-galactopyranoside (ONPG). The ONPG kit provides a quick and sensitive method for quantifying -galactosidase expression in cultured cells or tissues, while the CPRG kit is ideal for conditions in which β-galactosidse expression levels are low.

Contents
The -Galactosidase Assay Kits provide all the required reagents for 500 microassays in a 96-well plate and can be easily adapted for different assay sizes.
– 5X Lysis Buffer (1 vial x 55 ml)
– Standard Dilution Buffer (1 vial x 55 ml)
– ONPG Substrate Solution, 3mg/ml (o-nitrophenyl--D-galactopyranoside) (1 vial x 55 ml) OR 10X CPRG Substrate Stock (Chlorophenol red--D-galactopyranoside) (5 vials x 1 ml)
– Substrate Buffer (1 vial x 55 ml)
– Stop Buffer (1 vial x 55 ml)
– -galactosidase enzyme standard, 40 units (1 vial x 100 ml)

Shipping and Storage
Both -Galactosidase Assay Kits are shipped frozen.
The CPRG substrate solutions and -galactosidase enzyme standard should be stored at -20C.
All other components should be stored at 4C.
Stable for 6 months when stored properly.



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